Protein Info for GFF3361 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00694: Aconitase_C
  • amino acids 1 to 132 (132 residues), 135.8 bits, E=5.9e-44
• TIGR00171: 3-isopropylmalate dehydratase, small subunit
  • amino acids 1 to 196 (196 residues), 273.4 bits, E=4.9e-86

Best Hits

Swiss-Prot: 89% identical to LEUD_VARPS: 3-isopropylmalate dehydratase small subunit (leuD) from Variovorax paradoxus (strain S110)

KEGG orthology group: K01704, 3-isopropylmalate/(R)-2-methylmalate dehydratase small subunit [EC: 4.2.1.33 4.2.1.35] (inferred from 89% identity to vap:Vapar_1421)

MetaCyc: 61% identical to maleate hydratase small subunit (Pseudomonas alcaligenes NCIMB 9867)
Maleate hydratase. [EC: 4.2.1.31]

Predicted SEED Role

"3-isopropylmalate dehydratase small subunit (EC 4.2.1.33)" in subsystem Branched-Chain Amino Acid Biosynthesis or Leucine Biosynthesis (EC 4.2.1.33)

MetaCyc Pathways

• superpathway of branched chain amino acid biosynthesis (17/17 steps found)
• L-leucine biosynthesis (6/6 steps found)
• 3-methylbutanol biosynthesis (engineered) (6/7 steps found)
• L-isoleucine biosynthesis II (6/8 steps found)
• gentisate degradation II (3/4 steps found)
• 2,5-xylenol and 3,5-xylenol degradation (3/13 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Butanoate metabolism
• C5-Branched dibasic acid metabolism
• Valine, leucine and isoleucine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.33, 4.2.1.35

Use Curated BLAST to search for 4.2.1.31 or 4.2.1.33 or 4.2.1.35

Protein Sequence (216 amino acids)

>GFF3361 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MQKFTLHKGLVAPMDRENVDTDAIIPKQFLKSIRKTGFGQNLFDEWRYLDKGEPGQDPAS
RKPNPDFVLNQPRYAGASVLLARKNFGCGSSREHAPWAIDQYGFRAIIAPSYADIFFNNC
FKNGLLPIQLSEAQVAQLFDEVAAFPGYQLTIDLERQVIVKPQGDEIPFDVQAFRKYCLL
NGLDDIGLTLRYKDKIAAFEAERLATKPWLAHTMAA