Protein Info for GFF336 in Pseudomonas sp. DMC3

Annotation: Polyphosphate kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 741 transmembrane" amino acids 468 to 484 (17 residues), see Phobius details TIGR03705: polyphosphate kinase 1" amino acids 57 to 730 (674 residues), 942.6 bits, see alignment E=6.6e-288 PF13089: PP_kinase_N" amino acids 57 to 162 (106 residues), 123.3 bits, see alignment E=1.3e-39 PF02503: PP_kinase" amino acids 173 to 354 (182 residues), 205.9 bits, see alignment E=1.1e-64 PF17941: PP_kinase_C_1" amino acids 381 to 544 (164 residues), 232.3 bits, see alignment E=6.3e-73 PF13090: PP_kinase_C" amino acids 553 to 721 (169 residues), 222.7 bits, see alignment E=5.5e-70

Best Hits

Swiss-Prot: 92% identical to PPK1_PSESM: Polyphosphate kinase (ppk) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: K00937, polyphosphate kinase [EC: 2.7.4.1] (inferred from 98% identity to pfo:Pfl01_5464)

Predicted SEED Role

"Polyphosphate kinase (EC 2.7.4.1)" in subsystem High affinity phosphate transporter and control of PHO regulon or Phosphate metabolism or Purine conversions (EC 2.7.4.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.4.1

Use Curated BLAST to search for 2.7.4.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (741 amino acids)

>GFF336 Polyphosphate kinase (Pseudomonas sp. DMC3)
MNTEGLTEVAVKEAQPVVEQITETPPELEPAPPAPVAETAVAVPALAIPGLDDSSLYIHR
ELSQLQFNIRVLEQALDESYPLLERLKFLLIFSSNLDEFFEIRVAGLKKQITFAREQAGA
DGLQPHQALARISELVHGHVDRQYAILNDILLPELEKHQVRFIRRRNWTTKLKTWVRRYF
RDEIAPIITPIGLDPTHPFPLLVNKSLNFIVELEGIDAFGRDSGLAIIPAPRLLPRIIKV
PEEVGGAGDNYVFLSSMIHAHADDLFQGMKVKGCYQFRLTRNADLALDSEDVEDLARALR
GELFSRRYGDAVRLEVADTCPKHLSDYLLKQFNLSETELYQVNGPVNLTRLFSITGLDSH
PELQYTPFTPQIPKLLQNSENIFSVVSKQDILLLHPFESFTPVVDLLRQAAKDPHVLAVR
QTLYRSGANSEIVDALVDAARNGKEVTAVIELRARFDEESNLQLASRLQAAGAVVIYGVV
GFKTHAKMMLILRREAGEIVRYAHLGTGNYHAGNAKLYTDYSLLTSDDALCEDVGKLFSQ
LIGMGKTLRMKKLLHAPFTLKKGMLDMIARETQFALEGKPAHIIAKFNSLTDPKIIRALY
KASQSGVRIDLVVRGMCCLRPGIAGVSHNIHVRSIIGRFLEHTRVFYFLNGGDEQMFLSS
ADWMERNLDKRVETCFPVEGKKLLTRVKKELELYLTDNTHSWSLQSDGRYIRNTPTGNQN
PRSAQATLLERLGSPILPVSS