Protein Info for GFF3356 in Pseudomonas sp. DMC3

Annotation: Copper-exporting P-type ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 797 transmembrane" amino acids 157 to 175 (19 residues), see Phobius details amino acids 187 to 207 (21 residues), see Phobius details amino acids 219 to 236 (18 residues), see Phobius details amino acids 248 to 266 (19 residues), see Phobius details amino acids 403 to 422 (20 residues), see Phobius details amino acids 429 to 454 (26 residues), see Phobius details amino acids 743 to 761 (19 residues), see Phobius details amino acids 767 to 787 (21 residues), see Phobius details TIGR00003: copper ion binding protein" amino acids 8 to 68 (61 residues), 33.4 bits, see alignment 1.1e-11 amino acids 73 to 135 (63 residues), 34.3 bits, see alignment 5.3e-12 PF00403: HMA" amino acids 10 to 66 (57 residues), 47.2 bits, see alignment 3.5e-16 amino acids 75 to 133 (59 residues), 48.3 bits, see alignment 1.7e-16 TIGR01511: copper-translocating P-type ATPase" amino acids 201 to 786 (586 residues), 598.9 bits, see alignment E=2.7e-183 TIGR01525: heavy metal translocating P-type ATPase" amino acids 219 to 785 (567 residues), 631.8 bits, see alignment E=3.2e-193 TIGR01494: HAD ATPase, P-type, family IC" amino acids 256 to 765 (510 residues), 280.8 bits, see alignment E=4.2e-87 PF00122: E1-E2_ATPase" amino acids 284 to 463 (180 residues), 190 bits, see alignment E=4.4e-60 PF00702: Hydrolase" amino acids 482 to 697 (216 residues), 109.8 bits, see alignment E=3.5e-35

Best Hits

KEGG orthology group: K01533, Cu2+-exporting ATPase [EC: 3.6.3.4] (inferred from 91% identity to pfo:Pfl01_0661)

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (797 amino acids)

>GFF3356 Copper-exporting P-type ATPase (Pseudomonas sp. DMC3)
MSESTTFDLPIAGMTCASCAGRVERALRKVSGASAVSVNLATEQARVQAPGDSLPALMQA
VERAGYSVPRHTVELSIDGMTCASCVGRVERALNKVPSVSSVSVNLANERAHLELLGQVD
TQILLDAVSKAGYSASVWQAERPQSDDQQKRLHRERWALICAIALALPLVLPMLLQPFGI
HWMLPAWAQFALASPVQFIFGARFYVAAWKAVRAGAGNMDLLVALGTSAGYGLSVYEWAS
AAGRMPHLYFEASAVVIALVLLGKYLESRAKRQTASAIRALEALRPERAIQVIDGREQDV
AISALRLDDLVLVKPGERFPVDGEVVEGQSHADEALISGESLPVPKQPGDKVTGGAINGE
GRLLVRTQALGAESVLARIIRLVEDAQAAKAPIQKLVDKVSQIFVPTVLLIALATLIGWW
LHGAPLETAVINAVAVLVIACPCALGLATPTAIMAGTGVAARHGILIKDAEALERAHEVS
SVVFDKTGTLTSGTPRIAHFSAVDGDENNLLTLAGALQRGSEHPLAKAVLDAAAERGLNV
PDVSDSQSLTGRGIAGTLDGRRLALGNRRMLDESALSGGELSTSAEAWEREGRTLSWLIE
QSPQPKVLGLFAFGDTLKPGALQAVQQLAARDIQSHLLTGDNRGSARVVAEALGIQNVHA
EVLPADKAATVAELKKTGVVAMVGDGINDAPALAAADIGIAMGGGTDVAMHAAGITLMRG
DPRLVPAALEISRKTYAKIRQNLFWAFVYNLIGIPLAVFGFLNPVLAGAAMALSSVSVVS
NALLLKTWKPKDLEEHR