Protein Info for GFF3354 in Sphingobium sp. HT1-2

Annotation: Uncharacterized amino acid permease, GabP family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 520 transmembrane" amino acids 26 to 48 (23 residues), see Phobius details amino acids 58 to 81 (24 residues), see Phobius details amino acids 90 to 131 (42 residues), see Phobius details amino acids 152 to 171 (20 residues), see Phobius details amino acids 179 to 201 (23 residues), see Phobius details amino acids 211 to 232 (22 residues), see Phobius details amino acids 251 to 275 (25 residues), see Phobius details amino acids 330 to 351 (22 residues), see Phobius details amino acids 380 to 401 (22 residues), see Phobius details amino acids 408 to 425 (18 residues), see Phobius details amino acids 438 to 457 (20 residues), see Phobius details amino acids 463 to 480 (18 residues), see Phobius details PF13520: AA_permease_2" amino acids 25 to 460 (436 residues), 160.7 bits, see alignment E=6e-51 PF00324: AA_permease" amino acids 30 to 456 (427 residues), 124 bits, see alignment E=7.1e-40

Best Hits

KEGG orthology group: K03294, basic amino acid/polyamine antiporter, APA family (inferred from 81% identity to pzu:PHZ_c1901)

Predicted SEED Role

No annotation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (520 amino acids)

>GFF3354 Uncharacterized amino acid permease, GabP family (Sphingobium sp. HT1-2)
MIFGRVKPLDAILATAEKKSLHRSLGAFQLTMLGIGAVIGTGIFVLTAEAAQKAGPGMML
SFVIAGFVCAVAALCYAEMAAMVPVSGSAYTYSYAVMGELIAWMVGWALILEYAVAAGAV
SVGWSGYVVGLIEHTFHLDIPDLLTRGPFDGGMVNLPAMLIASLVTWLLVIGTKESATVN
AVLVAIKVSALTLFIVLSVPVMNMKGFTPFMPLGFSGVSAAAASIFFAYVGFDAVSTAAE
ETKNPQRNMPIGLIGSLAICTIFYMLVAAGVIGTVGAQPVYGPAGEVLAPGSTALSQQCA
ALAAAGTEAVTCSKEALAWTLREIGWPQIGNLLGLAAGLALPSVILMMMFGQTRIFFVMS
RDGLLPELFSKVHPTYKTPHVITILTGIFVALFAAFFPVGILADISNSGTLFAFAAVSIA
VMRLRKTDADRKRPFRTPAIYITAPIAIAGCAYLFWSLGVETKLMFVGWATLGLIVYFAY
SRSRSHVGRGIVDVAEAGAGIPPQPVPPLPGAHTPGGQDA