Protein Info for GFF3336 in Variovorax sp. SCN45

Annotation: tRNA-5-carboxymethylaminomethyl-2-thiouridine(34) synthesis protein MnmG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 649 transmembrane" amino acids 609 to 628 (20 residues), see Phobius details TIGR00136: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA" amino acids 7 to 630 (624 residues), 905.4 bits, see alignment E=8.3e-277 PF12831: FAD_oxidored" amino acids 8 to 154 (147 residues), 35.9 bits, see alignment E=1.4e-12 PF00890: FAD_binding_2" amino acids 8 to 38 (31 residues), 21.9 bits, see alignment (E = 2.3e-08) PF01134: GIDA" amino acids 8 to 405 (398 residues), 566.7 bits, see alignment E=7.1e-174 PF21680: GIDA_C_1st" amino acids 466 to 564 (99 residues), 88 bits, see alignment E=1.5e-28 PF13932: SAM_GIDA_C" amino acids 570 to 625 (56 residues), 85.9 bits, see alignment 3.3e-28

Best Hits

Swiss-Prot: 97% identical to MNMG_VARPS: tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG) from Variovorax paradoxus (strain S110)

KEGG orthology group: K03495, glucose inhibited division protein A (inferred from 97% identity to vpe:Varpa_0050)

Predicted SEED Role

"tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (649 amino acids)

>GFF3336 tRNA-5-carboxymethylaminomethyl-2-thiouridine(34) synthesis protein MnmG (Variovorax sp. SCN45)
MLYPEEFDVIVVGGGHAGTEAALAAARMGAKTLLLSHNIETLGQMSCNPSIGGIGKGHLV
KEVDALGGAMAIATDEAGIQFRILNSSKGPAVRATRAQADRVLYKAAIRHRLENQPNLSL
FQQAVDDLMVEGDRVVGAVTQVGIAFRARTVVLTAGTFLDGRIHVGLDNYQAGRAGDPPA
VSLSARLKELKLPQGRLKTGTPPRLDGRSIDFSKCTEQPGDGMPGGAGPMPVFSFMGRTD
MHPRQMPCWITHTNARTHEIIRSGFDRSPMFTGKIDGVGPRYCPSVEDKINRFADKESHQ
IFLEPEGLTTNEYYPNGISTSLPFDIQYQLVRSMPGLENAHILRPGYAIEYDYFDPRELK
SSFETRSVKGLFFAGQINGTTGYEEAAAQGLFAGINAALQCRGEEAWLPRRDEAYLGVLV
DDLITKGVTEPYRMFTSRAEFRLQLREDNADMRLTEAGRKLGLVDDARWDAFSRKRDAVS
RETERLKSIWVNPRNLPASESERVLGKVIDHEYNLADLLRRPDVNYETLMSLDGSKYAAP
VALSETEIEQIEISAKYSGYIERQHDEVERAAHFENLKLPADFDYGQVKALSFEVRQKLD
KHRPETLGLASRISGVTPAAISLLMIHLRKGGHKAFSRDAATTTEAAVE