Protein Info for GFF3324 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Mannose-1-phosphate guanylyltransferase (GDP) (EC 2.7.7.22)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01479: mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase
  • amino acids 1 to 453 (453 residues), 556.5 bits, E=2.5e-171
• PF00483: NTP_transferase
  • amino acids 5 to 269 (265 residues), 115.6 bits, E=5.7e-37
• PF22640: ManC_GMP_beta-helix
  • amino acids 282 to 331 (50 residues), 45.1 bits, 1.6e-15
• PF01050: MannoseP_isomer
  • amino acids 339 to 449 (111 residues), 182.8 bits, E=4.2e-58
• PF07883: Cupin_2
  • amino acids 367 to 433 (67 residues), 43.7 bits, E=3.5e-15

Best Hits

KEGG orthology group: K00971, mannose-1-phosphate guanylyltransferase [EC: 2.7.7.22] K01809, mannose-6-phosphate isomerase [EC: 5.3.1.8] (inferred from 67% identity to pna:Pnap_3192)

Predicted SEED Role

"Mannose-1-phosphate guanylyltransferase (GDP) (EC 2.7.7.22)" in subsystem Alginate metabolism or Mannose Metabolism (EC 2.7.7.22)

MetaCyc Pathways

• colanic acid building blocks biosynthesis (10/11 steps found)
• GDP-mannose biosynthesis (3/4 steps found)
• β-1,4-D-mannosyl-N-acetyl-D-glucosamine degradation (2/3 steps found)
• D-mannose degradation I (1/2 steps found)
• D-mannose degradation II (1/2 steps found)
• mannitol degradation II (2/4 steps found)
• mannitol biosynthesis (1/3 steps found)
• 1,5-anhydrofructose degradation (2/5 steps found)
• superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis (8/14 steps found)
• β-(1,4)-mannan degradation (2/7 steps found)

KEGG Metabolic Maps

• Fructose and mannose metabolism
• Nucleotide sugars metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.22, 5.3.1.8

Use Curated BLAST to search for 2.7.7.22 or 5.3.1.8

Protein Sequence (461 amino acids)

>GFF3324 Mannose-1-phosphate guanylyltransferase (GDP) (EC 2.7.7.22) (Hydrogenophaga sp. GW460-11-11-14-LB1)
LSRQSFPKQFVPLVGDKSLLQLTLDRLAWCDNPVICVAAEDHRFLVADAIEAENVPGQVL
LEPAARNTAAAMALAALLARAKDNADPLLLFCPSDHHIPDAAAFAETVRQGVPAAEAGAI
VTFGVMPSFPSSAYGYIQQGDHRADGSRKVARFIEKPAPAAAQALLLQGNVLWNAGIFLA
RASTLLAALGQHAADILQACEQAMAKTRQDQVGQIHFVRPEAEPFLACRSQSIDYAVMEP
HDDVAVVPFQGQWSDVGSWNAVAELSTADGEGNRVTGQGHALNARNTFIHAPDRTVVALG
TQDLLIIDTPDALLVAQRDHAEQVKDVVAHLSQRKVPQAAMHRRVARPWGWYDSVDMGER
FQVKRIGVKPGASLSLQKHHHRAEHWIIVKGTAEVTRGQEVYLLSENQSTYIPIGEVHRL
HNPGKMVLEMIEVQSGSYLGEDDIVRLEDIYGRQPDPANKK