Protein Info for GFF3318 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: S-(hydroxymethyl)glutathione dehydrogenase (EC 1.1.1.284)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02818: S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase
  • amino acids 2 to 368 (367 residues), 622.5 bits, E=1.2e-191
• PF08240: ADH_N
  • amino acids 28 to 123 (96 residues), 83.7 bits, E=7.9e-28
• PF00107: ADH_zinc_N
  • amino acids 197 to 328 (132 residues), 109.7 bits, E=1e-35

Best Hits

Swiss-Prot: 76% identical to FADH_PARDE: S-(hydroxymethyl)glutathione dehydrogenase (flhA) from Paracoccus denitrificans

KEGG orthology group: K00121, S-(hydroxymethyl)glutathione dehydrogenase / alcohol dehydrogenase [EC: 1.1.1.1 1.1.1.284] (inferred from 94% identity to vap:Vapar_1703)

MetaCyc: 76% identical to FlhA (Paracoccus denitrificans)
S-(hydroxymethyl)glutathione dehydrogenase. [EC: 1.1.1.284]

Predicted SEED Role

"S-(hydroxymethyl)glutathione dehydrogenase (EC 1.1.1.284)" in subsystem Glutathione-dependent pathway of formaldehyde detoxification (EC 1.1.1.284)

MetaCyc Pathways

• phytol degradation (4/4 steps found)
• 3-methylbutanol biosynthesis (engineered) (6/7 steps found)
• ethanol degradation II (3/3 steps found)
• formaldehyde oxidation II (glutathione-dependent) (3/3 steps found)
• ethanolamine utilization (4/5 steps found)
• pyruvate fermentation to isobutanol (engineered) (4/5 steps found)
• superpathway of anaerobic sucrose degradation (14/19 steps found)
• acetaldehyde biosynthesis I (1/1 steps found)
• L-isoleucine degradation II (2/3 steps found)
• L-leucine degradation III (2/3 steps found)
• L-valine degradation II (2/3 steps found)
• ethanol degradation I (1/2 steps found)
• pyruvate fermentation to ethanol II (1/2 steps found)
• mixed acid fermentation (11/16 steps found)
• (S)-propane-1,2-diol degradation (3/5 steps found)
• acetylene degradation (anaerobic) (3/5 steps found)
• protein S-nitrosylation and denitrosylation (3/5 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (13/19 steps found)
• L-phenylalanine degradation III (2/4 steps found)
• L-tyrosine degradation III (2/4 steps found)
• salidroside biosynthesis (2/4 steps found)
• heterolactic fermentation (12/18 steps found)
• serotonin degradation (4/7 steps found)
• L-methionine degradation III (1/3 steps found)
• pyruvate fermentation to ethanol I (1/3 steps found)
• pyruvate fermentation to ethanol III (1/3 steps found)
• noradrenaline and adrenaline degradation (8/13 steps found)
• phenylethanol biosynthesis (2/5 steps found)
• superpathway of fermentation (Chlamydomonas reinhardtii) (4/9 steps found)
• butanol and isobutanol biosynthesis (engineered) (3/8 steps found)
• superpathway of N-acetylneuraminate degradation (13/22 steps found)
• superpathway of C1 compounds oxidation to CO₂ (4/12 steps found)
• superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (7/17 steps found)
• superpathway of Clostridium acetobutylicum solventogenic fermentation (4/13 steps found)
• L-tryptophan degradation V (side chain pathway) (1/13 steps found)

KEGG Metabolic Maps

• 1- and 2-Methylnaphthalene degradation
• 3-Chloroacrylic acid degradation
• Drug metabolism - cytochrome P450
• Fatty acid metabolism
• Glycine, serine and threonine metabolism
• Glycolysis / Gluconeogenesis
• Metabolism of xenobiotics by cytochrome P450
• Methane metabolism
• Retinol metabolism
• Tyrosine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.1

Use Curated BLAST to search for 1.1.1.1 or 1.1.1.284

Protein Sequence (368 amino acids)

>GFF3318 S-(hydroxymethyl)glutathione dehydrogenase (EC 1.1.1.284) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MKTQAAVAWKAGQPLTIETVDLQGPKFGEVLVEIKATGICHTDYYTLSGADPEGIFPAIL
GHEGAGIVVDVGPGVTTLKKGDHVIPLYTPECRQCKFCLSRKTNLCQLIRGTQGKGLMPD
ATSRFSLDGKPIFHYMGTSTFSNYTVAPEISLAKIREDAPFDKVCYIGCGVTTGIGAVIF
TAKVEAGANVVVFGLGGIGLNVIQGAKMVGADKIIGVDLNPARQEMARKFGMTHFINPKE
VENVVDAIVQLTDGGADYSFECIGNTQVMRQALECTHKGWGRSIIIGVAEAGAEISTRPF
QLVTGRKWEGSAFGGARGRTDVPKIVDWYMDGKINIDDLITHKLKLADINEGFALMKRGE
SIRAVVEF