Protein Info for GFF3308 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: N-carbamoyl-L-amino acid hydrolase (EC 3.5.1.87)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 597 PF09349: OHCU_decarbox" amino acids 9 to 170 (162 residues), 163.2 bits, see alignment E=1.1e-51 TIGR03164: OHCU decarboxylase" amino acids 18 to 171 (154 residues), 169.2 bits, see alignment E=7.4e-54 TIGR01879: amidase, hydantoinase/carbamoylase family" amino acids 189 to 583 (395 residues), 316 bits, see alignment E=3.8e-98 PF01546: Peptidase_M20" amino acids 256 to 587 (332 residues), 64.5 bits, see alignment E=2e-21

Best Hits

KEGG orthology group: K06016, N-carbamoyl-L-amino-acid hydrolase [EC: 3.5.1.87] (inferred from 76% identity to ajs:Ajs_1115)

Predicted SEED Role

"N-carbamoyl-L-amino acid hydrolase (EC 3.5.1.87)" in subsystem Hydantoin metabolism (EC 3.5.1.87)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.1.87

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (597 amino acids)

>GFF3308 N-carbamoyl-L-amino acid hydrolase (EC 3.5.1.87) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MPITLEQLNRAPPAEAAQMLDGLYEHSPWIAEQALARRPFASLAALKHAMVAVLDQAGLE
PQLALIRAHPELAGKAMVAKSLTAESTNEQTKAGLTNCTPDEFAHIQQLNAQYNAKFGFP
FILAVRGPRGVGLNKREIIATFERRLGHPVDFERAECLRNIHRIAEIRLNDKFGFEPTVG
HRVWDWHEDLARHSDPGFAEHGQLTVTYLTDAHRACATDIAARMRDCGFDEVHIDAVGNV
VGRYHGATPGAPALLTGSHYDTVRNGGKYDGRLGIFAPMACVQQLHAAGRRLPFAFEVVG
FAEEEGQRYKATFLGSGALTGHFNPAWLDQLDADGVSMRDAMRHAGLPATLEAIAALQRD
PSKYLGFVEVHIEQGPVLNEMDLPLAVVTSINASVRYQCEALGMASHAGTTPMDRRRDAA
SAVAELALYAEQRAAADGDSVATIGMLQVPGGSINVVPGRCLFSLDLRAPSNAQRDALER
DILAQLRSICERRGIGLKTEETMRAAAAPSAPAWQARWEKAVAALGVPLHRMPSGAGHDA
MKLHEVMPQAMLFVRGLNSGISHNPLESSTSDDMELCVNAFSHLLEQLATEHPNGTP