Protein Info for PS417_16915 in Pseudomonas simiae WCS417

Annotation: hemolysin D

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 399 transmembrane" amino acids 23 to 43 (21 residues), see Phobius details PF13533: Biotin_lipoyl_2" amino acids 62 to 110 (49 residues), 26.7 bits, see alignment 7.2e-10 PF16576: HlyD_D23" amino acids 64 to 292 (229 residues), 55.1 bits, see alignment E=1.3e-18 PF00529: CusB_dom_1" amino acids 203 to 347 (145 residues), 37.6 bits, see alignment E=3.5e-13 PF13437: HlyD_3" amino acids 218 to 298 (81 residues), 51.1 bits, see alignment E=4.2e-17

Best Hits

Swiss-Prot: 46% identical to EMRA_ECOLI: Multidrug export protein EmrA (emrA) from Escherichia coli (strain K12)

KEGG orthology group: K03543, multidrug resistance protein A (inferred from 96% identity to pfs:PFLU3876)

MetaCyc: 46% identical to multidrug efflux pump membrane fusion protein EmrA (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-363; TRANS-RXN-364; TRANS-RXN-365

Predicted SEED Role

"Membrane fusion component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7U8E8 at UniProt or InterPro

Protein Sequence (399 amino acids)

>PS417_16915 hemolysin D (Pseudomonas simiae WCS417)
MATAENTNATEQPKDNNPRKRKVMLIGLALIVVLAVVGVWGWYELYGRFNESTDDAYVNG
NVVEITPLVTGTVVSIGADDGDLVHEGQVLVNFDPNDAAVGLQSAQANLARTVRQVRGLY
SNVDGMKAQVNAQKADVQTAQDNFNRRKALAQGGAISQEELSHARDSLTAAKNALTNLEQ
QLKTTNALVDDTVISSHPDVQAAAAQLRQAYLTNARSTLIAPVTGYVAKRTVQLGQRVQP
GTALMAVIPLNQLWIDANFKETQLRDMRIGQPVDIESDIYGSDVKFSGTIDSLGAGTGSA
FALLPAQNATGNWIKIVQRVPVRIHINADELAKHPLRVGLSTLVNVDLHDQSGPVLAQQA
PQKASFTTNVYDRQLAEADAMINQLIHDNSLAAPKAVQR