Protein Info for GFF3299 in Sphingobium sp. HT1-2

Annotation: NAD synthetase (EC 6.3.1.5) / Glutamine amidotransferase chain of NAD synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00795: CN_hydrolase
  • amino acids 7 to 241 (235 residues), 109 bits, E=2.6e-35
• PF02540: NAD_synthase
  • amino acids 268 to 526 (259 residues), 232.6 bits, E=3.9e-73
• TIGR00552: NAD+ synthetase
  • amino acids 275 to 529 (255 residues), 192 bits, E=5.2e-61

Best Hits

Swiss-Prot: 55% identical to NADE_RHOCA: Glutamine-dependent NAD(+) synthetase (nadE) from Rhodobacter capsulatus

KEGG orthology group: K01916, NAD+ synthase [EC: 6.3.1.5] (inferred from 90% identity to sjp:SJA_C1-03210)

Predicted SEED Role

"NAD synthetase (EC 6.3.1.5) / Glutamine amidotransferase chain of NAD synthetase" in subsystem NAD and NADP cofactor biosynthesis global or NAD regulation (EC 6.3.1.5)

MetaCyc Pathways

• aspartate superpathway (25/25 steps found)
• NAD de novo biosynthesis I (6/6 steps found)
• NAD de novo biosynthesis III (6/6 steps found)
• NAD biosynthesis from 2-amino-3-carboxymuconate semialdehyde (4/4 steps found)
• NAD de novo biosynthesis IV (anaerobic) (5/6 steps found)
• NAD salvage pathway II (PNC IV cycle) (4/5 steps found)
• NAD salvage pathway I (PNC VI cycle) (5/7 steps found)
• NAD salvage pathway V (PNC V cycle) (3/5 steps found)
• NAD de novo biosynthesis II (from tryptophan) (5/9 steps found)
• NAD salvage (plants) (5/11 steps found)
• superpathway of NAD biosynthesis in eukaryotes (7/14 steps found)

KEGG Metabolic Maps

• Nicotinate and nicotinamide metabolism
• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.1.5

Use Curated BLAST to search for 6.3.1.5

Protein Sequence (553 amino acids)

>GFF3299 NAD synthetase (EC 6.3.1.5) / Glutamine amidotransferase chain of NAD synthetase (Sphingobium sp. HT1-2)
MTDKLVIALAQMTQTMGDPRANADAMLEWRARAKGADLIVFPELQLIGYPPEDLVLKPAL
LDQANYHLDRLAQESADGGPAMLVGTVVASQGVLFNVVALIENGAVTAIRQKRELPNYGT
FDEKRLFAPGPLPAPIDFRGVKIGVPICEDIWFPFVTAHLRAEGAEILISPNGSPFEVDK
DDRRLNVVAGTRVRETGLPLVYLNRVGGQDELVFDGASFVMNGDLSIAQQLPDWEEKLVL
TQWEKWDGQWVCLPGERHDLDPRPADIYNAMLLGLRDYVNRNRFPGVVLGLSGGIDSALS
AAVAVDALGADRVWCVMMPSRFTSQESLDDAVECARLLGVRYDTIPIEPAVAAFDTMLAP
VFEGKARDLTEENIQSRIRGLTLMALSNKFGHMLLTTGNKSEMSVGYATIYGDMAGGYSV
LKDAYKTTVFDLCRWRNDNMPSLGEGFGPAGPVMPERVITKPPSAELRDNQKDEDSLPPY
EVLDPILYGLVEEELSVAQLVERGFDKAVVTRIEHLLYIAEYKRRQSPPGVKLGIRNFGR
DRRYPITNAFRSV