Protein Info for GFF3290 in Xanthobacter sp. DMC5
Annotation: hypothetical protein
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K02230, cobaltochelatase CobN [EC: 6.6.1.2] (inferred from 83% identity to xau:Xaut_3286)Predicted SEED Role
"CobN component of cobalt chelatase involved in B12 biosynthesis" in subsystem Coenzyme B12 biosynthesis
MetaCyc Pathways
- cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation) (12/13 steps found)
- adenosylcobalamin biosynthesis II (aerobic) (26/33 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 6.6.1.2
Use Curated BLAST to search for 6.6.1.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (1110 amino acids)
>GFF3290 hypothetical protein (Xanthobacter sp. DMC5) MHILATTSTSLEELAEPVDLAQPPGDMVALSFTDSDLIGLSAAWRARGDAVPSLRLARLK DLRHPMSVDLWVEKVAAKVKVVLVRQLGGLDWWRYGAEQLAAMARARGIALALLPGEDQD DPRLAALSTLPQDTLALWLGYFRAGGPENMAALLAAMGEAAGFPAPHAIPAPHPVPATAF HRPGAGLATLEEVRAVGRPNALILFYRSQWLAADTAAVDALAEALAARGIAAHCLFVPSL KAPDARAAVRAAAAALNPAIILTTTAFAAGEAAELFPQGGSPVLQAAPATTAREAWAGSP RGFSAADLAMHVVLPELDGRVLAGALSFKDFAPPDAELAFTAAINRPEPEAVAHVADRAA ALVRLAATPRAERRLALILPDYPGAEGRSGYAVGLDVPESARRMLGTLAAAGYGTGPVPD DAKALLAAFRPEPALTLEVYRRLFAALPEAAQAAVTAAWGAPEDDADVSDGAFRFRLACS GTITLAFAPDRGANLDRRADYHDASLPPRHGLLAFGLWLRAGLDAHAVVHLGAHGTLEWL PGKAVALTPACFPQVVMGALPVVYPFIVSNPGEAAQAKRRLAAVTIGHLPPPLASAGLSE AEQRLERLVDEYAQADGLDRRRRERLAGLIVETAAETGLAATAGVSAATDPDEALRRIDA WLCDLKDLAVKDGLHIFGAMAEGEADPLRAASAEAENRALLTALDGRFVPAGPAGAPARG RRDVLPTGRNLFTADPRTLPTPTACDLARKAADEALRLILQEQGDWPRALVLDLWGSASL RTGGEEIAQGLAFLGCRPVWEPATGRVTGVEVLPPAVLGRPRIDVTFRISGLFRDIFPAQ IALLDAAVRAVAARDEEESENPLAEARRKGEDLSRLFGSAPGAYGAGIEANLQRGAFETR EELGHAYLAAASHAYGGADGVAHPAPGLFAARLAEADALVHGADDAARDLLDGSEDAAFM GGFAAAGAALGRSAHLVVLDTTDPDRPRARPLKEALTRLVYGRVSARFIAGQMRHGPRGA SELAETVDRLVAFAETTEQVPSALLDRLHAAYLGDPAVRAFLLAQNPDAARAIARRFADA RRRGLWHARRNDLDADLDALTAEAGREAAE