Protein Info for GFF3272 in Variovorax sp. SCN45

Annotation: Inner membrane protein, KefB/KefC family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 661 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details transmembrane" amino acids 55 to 74 (20 residues), see Phobius details amino acids 84 to 110 (27 residues), see Phobius details amino acids 117 to 141 (25 residues), see Phobius details amino acids 154 to 175 (22 residues), see Phobius details amino acids 182 to 203 (22 residues), see Phobius details amino acids 223 to 256 (34 residues), see Phobius details amino acids 275 to 293 (19 residues), see Phobius details amino acids 300 to 323 (24 residues), see Phobius details amino acids 334 to 351 (18 residues), see Phobius details amino acids 360 to 383 (24 residues), see Phobius details TIGR00932: transporter, monovalent cation:proton antiporter-2 (CPA2) family" amino acids 11 to 290 (280 residues), 242.3 bits, see alignment E=3.5e-76 PF00999: Na_H_Exchanger" amino acids 11 to 375 (365 residues), 199.3 bits, see alignment E=1.4e-62 PF02254: TrkA_N" amino acids 416 to 529 (114 residues), 85.9 bits, see alignment E=3.9e-28 PF02080: TrkA_C" amino acids 592 to 657 (66 residues), 47 bits, see alignment E=3e-16

Best Hits

KEGG orthology group: K03455, monovalent cation:H+ antiporter-2, CPA2 family (inferred from 94% identity to vpe:Varpa_6016)

Predicted SEED Role

"Glutathione-regulated potassium-efflux system protein KefB" in subsystem Glutathione-regulated potassium-efflux system and associated functions

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (661 amino acids)

>GFF3272 Inner membrane protein, KefB/KefC family (Variovorax sp. SCN45)
MSSFDLTLLYLLAAVIGVVACRSLKLPPMLGYLSAGVLIGPHAFALAQNSEGIRHLGEFG
VVFLMFVIGLEFSLPKLRAMRKHVFGLGLLQVLLTMTIATVGALLIARLLPPSWQLGWQT
ALALSGALTMSSTAIVVKLMAERLELESEHGKRVMGVLLFQDLAVVPLLVLIPALGAPPE
ALAKALGLALLKATFLIGVLLYGGPRVMRWWLTLVARRRSEELFILNVLLVTLGLAWLTE
LAGLSLALGAFIAGMLVSETEYKHQVETDIRPFHDVLLGLFFITVGMSLDWHIVVERWML
VGVLLLVPLGFKLGLVTVLARVLGATSGVSLRTGLYLAQAGEFGFVLLTLAQERSLLPPW
LANPVLASMVLSMLATPFIIMYSNAIVRKLVASDWLQQSLQMTSIARKTINTAQHVIICG
YGRCGQNLARILEREGIPYMALDLDPDRVRQAAAAGDSVVFGDAARLQALMAAGLARASA
VVVTYLDVPGAMKVLANARAHAAHVPVIVRTQDDHDLEKLQAAGATEVVPEAIEGSLMLA
SHALALVGVPMRRVIRVVQDQRDARYNLLRGYFHGADDDKADEVDHERLNSFTLSAGARA
IGQTLEQLALPTLGVRVASLRRHDGQPRTPGADTVLTDGDTLVLSGKPTALAVAIERLQK
G