Protein Info for GFF327 in Variovorax sp. SCN45

Annotation: 5'-methylthioadenosine nucleosidase (EC 3.2.2.16) @ S-adenosylhomocysteine nucleosidase (EC 3.2.2.9)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01048: PNP_UDP_1
  • amino acids 53 to 322 (270 residues), 144.6 bits, E=1.7e-46

Best Hits

KEGG orthology group: K01243, S-adenosylhomocysteine/5'-methylthioadenosine nucleosidase [EC: 3.2.2.9] (inferred from 87% identity to vpe:Varpa_3249)

Predicted SEED Role

"5'-methylthioadenosine nucleosidase (EC 3.2.2.16) @ S-adenosylhomocysteine nucleosidase (EC 3.2.2.9)" (EC 3.2.2.16, EC 3.2.2.9)

MetaCyc Pathways

• S-methyl-5'-thioadenosine degradation IV (1/1 steps found)
• S-adenosyl-L-methionine salvage I (3/4 steps found)
• autoinducer AI-2 biosynthesis II (Vibrio) (4/6 steps found)
• S-methyl-5'-thioadenosine degradation I (1/2 steps found)
• autoinducer AI-2 biosynthesis I (3/5 steps found)
• L-cysteine biosynthesis VI (reverse transsulfuration) (4/7 steps found)
• 5'-deoxyadenosine degradation II (1/4 steps found)
• L-methionine salvage cycle I (bacteria and plants) (6/12 steps found)
• L-methionine salvage cycle II (plants) (4/11 steps found)

KEGG Metabolic Maps

• Methionine metabolism
• Urea cycle and metabolism of amino groups

Isozymes

Compare fitness of predicted isozymes for: 3.2.2.16, 3.2.2.9

Use Curated BLAST to search for 3.2.2.16 or 3.2.2.9

Protein Sequence (325 amino acids)

>GFF327 5'-methylthioadenosine nucleosidase (EC 3.2.2.16) @ S-adenosylhomocysteine nucleosidase (EC 3.2.2.9) (Variovorax sp. SCN45)
MTTTTKNTSSLLRRLRAPVLAAAFGVLLAGCVGVYKSDAVSINSSTRLDDTPRIAVLSAF
EPELKLLLTRLQGPVRHTVNGVEFTTGTLEGKPVVLFLSGISMTNATMNTQLVLNRFRVT
NIVFSGIAGGVNPQLHVGDVTVPAQWGQYLEVLMARETAPGKYSAPPFITDATLPNFGMM
FPRPVEVRSAANPQIVRKFWFEADPKMLEVARSIHNVDLANCGAGKCLPRKPQLVVGGNG
VSGQAFMDNKAYREYTYKTFQANVLDMETAAVGMVAYSNGVPYIAFRSLSDLAGGGEGEN
EMGTFMSIAADNSAKVMLAFLAAWK