Protein Info for GFF3251 in Xanthobacter sp. DMC5

Annotation: 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxy late synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 493 signal peptide" amino acids 1 to 35 (35 residues), see Phobius details PF12146: Hydrolase_4" amino acids 123 to 339 (217 residues), 38.6 bits, see alignment E=1.2e-13 PF12697: Abhydrolase_6" amino acids 129 to 283 (155 residues), 43.5 bits, see alignment E=8.8e-15 PF00561: Abhydrolase_1" amino acids 129 to 241 (113 residues), 36.2 bits, see alignment E=8.1e-13

Best Hits

KEGG orthology group: None (inferred from 81% identity to xau:Xaut_1229)

Predicted SEED Role

"Putative membrane protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (493 amino acids)

>GFF3251 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxy late synthase (Xanthobacter sp. DMC5)
MGAVGFMWRGTKRTAKIVALLIFAVVATIFVVRAVDSQRGPPLDRWHTYHPPELSRSALA
TADLAAYMAAEDKAFAGVKANVTDKVDPEDRVRFNRYFASSPIYPPGFAQDFNRTYVLQP
KGEPAGAAVFLHGLTDSPYSHRHLAQLYADHGFIAIVLRLPGHGTVPGSLTQIGHEEWIA
ATALAMREARRRVGPGKPVHIVGYSNGGALALMHALDALDDPSLVAPSRIVLMSPMVGVT
EFARFAGLAGLPAIFPAFAKAAWLNITPEFNPFKYNSFPVHAARESFQLTQVLQANIERH
SRAGRLTGLAPVLTFQSVLDHTVSARAVIEQLHARLDDNGSELVLIDINRSGQFEDLLNR
RSDTAISSLLPPAPRRFRTAVIGNATPGSSATVVRETPAGATEETVRPLTAVYPEDVYSL
SHVAVPFPLSDGLYGLEPDPAEKFGIRLGDIAARGEYGALVVDVDMLVRMSSNPFFPYLE
ERVAAGLPPARAP