Protein Info for GFF3219 in Pseudomonas sp. DMC3

Annotation: tRNA dimethylallyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 323 signal peptide" amino acids 1 to 17 (17 residues), see Phobius details TIGR00174: tRNA dimethylallyltransferase" amino acids 7 to 305 (299 residues), 349.6 bits, see alignment E=6.3e-109 PF01715: IPPT" amino acids 41 to 294 (254 residues), 306.1 bits, see alignment E=2.1e-95

Best Hits

Swiss-Prot: 93% identical to MIAA_PSEPF: tRNA dimethylallyltransferase (miaA) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K00791, tRNA dimethylallyltransferase [EC: 2.5.1.75] (inferred from 93% identity to pfo:Pfl01_0522)

Predicted SEED Role

"tRNA dimethylallyltransferase (EC 2.5.1.75)" (EC 2.5.1.75)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.5.1.75

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (323 amino acids)

>GFF3219 tRNA dimethylallyltransferase (Pseudomonas sp. DMC3)
MSQLPPAIFLMGPTAAGKTDLAIELTKVLPCELISVDSALVYRGMDIGTAKPSKELLAQY
PHRLIDILDPAEAYSAADFRRDALQAMAEITGRGKIPLLVGGTMLYYKALVEGLADMPAA
DPEVRTQIEEEAARLGWQALHDQLAQIDPVSAARIHPNDPQRLSRALEVYRVSGQSMTAL
REQQSAQSTEAAASGRQQLPYTVANLAIAPANRQVLHQRIEQRFTNMLEQGFIDEVVALR
KRSDLHSGLPSIRAVGYRQVWDYLDGKLTLAQMQERGIIATRQLAKRQFTWLRSWDDLHW
LDSLDCDNLPRALKYLGTISILS