Protein Info for GFF3210 in Sphingobium sp. HT1-2

Annotation: Metallopeptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 680 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details PF05649: Peptidase_M13_N" amino acids 55 to 425 (371 residues), 410.1 bits, see alignment E=1.3e-126 PF01431: Peptidase_M13" amino acids 477 to 677 (201 residues), 211 bits, see alignment E=1.5e-66

Best Hits

KEGG orthology group: K07386, putative endopeptidase [EC: 3.4.24.-] (inferred from 81% identity to sch:Sphch_2390)

Predicted SEED Role

"Metallopeptidase"

Isozymes

Compare fitness of predicted isozymes for: 3.4.24.-

Use Curated BLAST to search for 3.4.24.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (680 amino acids)

>GFF3210 Metallopeptidase (Sphingobium sp. HT1-2)
MKFLLMGAAATGLALAATVAHADQPATTTAAAPAAKPTYGTYGFDTAGMDASVKPGDDFY
EYANGTWLKNTPIPADKSNYGAFTVLDELSQKRTREILDGAKTDPNSKIGVAYATYLDSA
AVEAKGLAPIKPWLAEIGAVKDLKAYAALSGKAARAGVRGPFRFYVGQDDKDPETYILSM
MQGGLGLPDRDYYLDQSEKMAAIRTAYVAHLEQMLTLLGEPNAKARAAALMAFETEIAKV
HWTQVDSRDADKTYNKMTLAALQKAAPGFDFAAYFGAVGLKPSDLLVAQPSAVTGEAALI
AKAPIGVLKDSLLLRSVHSYADYLPDNIASADFAFFGTTLSGTPEREERWKRGVTFLKES
LGEEVGKVYVAKYFPPETKAAMDQLVKNVLSAMGRRIDGLPWMSDEAKARAHKKLAAFTP
KIGYPDRWRDYSGLTIKSGDLFGNAMRSNQFDFDYNIGKLGKPIYRWEWGMTPMEINAYA
NFGMVEIVFPAAILQPPFFDPHADPAVNYGGIGAVIGHELSHHFDDQGAKYDETGKLNQW
WTDQDVANFKALTAKLGAQYDAYEPFPGAHVKGAFTMGENIGDLGGLAVALDAYHASLGG
KAAPVIDGMTGDQRFFLGWAQVWRRNYREANLRQRLVTDPHAPSQYRADIVRNFDAWYDA
FKPAPGGKIYLDPKDRVKIW