Protein Info for PS417_16370 in Pseudomonas simiae WCS417

Annotation: chemotaxis protein CheY

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 917 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details transmembrane" amino acids 28 to 29 (2 residues), see Phobius details amino acids 168 to 188 (21 residues), see Phobius details PF09984: sCache_4" amino acids 35 to 168 (134 residues), 65 bits, see alignment E=2e-21 PF00672: HAMP" amino acids 190 to 240 (51 residues), 51.4 bits, see alignment 3.3e-17 PF00512: HisKA" amino acids 285 to 349 (65 residues), 81.7 bits, see alignment 9.5e-27 PF02518: HATPase_c" amino acids 396 to 511 (116 residues), 94.7 bits, see alignment E=1.5e-30 PF00072: Response_reg" amino acids 669 to 783 (115 residues), 99.5 bits, see alignment E=3.9e-32 PF01627: Hpt" amino acids 830 to 911 (82 residues), 47.6 bits, see alignment E=4.9e-16

Best Hits

Swiss-Prot: 87% identical to GACS_PSEPH: Sensor histidine kinase GacS (gacS) from Pseudomonas protegens (strain DSM 19095 / LMG 27888 / CHA0)

KEGG orthology group: K07678, two-component system, NarL family, sensor histidine kinase BarA [EC: 2.7.13.3] (inferred from 99% identity to pfs:PFLU3777)

Predicted SEED Role

"BarA sensory histidine kinase (= VarS = GacS)" in subsystem Type III secretion system orphans

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UGW5 at UniProt or InterPro

Protein Sequence (917 amino acids)

>PS417_16370 chemotaxis protein CheY (Pseudomonas simiae WCS417)
MLTRMGIKGRVLLLTLLPTSLMASLLGGYFTWMQLSELQTQLLQRGEMIAEQLAPLVAPA
LSARNTELLERIATQSLEQPDVRAVSFLSPERAPLAHAGPTMLNQPPVGNSSHLLQRTGN
DATRYLLPVFGRHRNLAGELIPDEADRLLGWVEVELSHNGMLLRGYRSLFASLLLIAIGL
ICTAALALRISRTINSPIGQIKQAVAQLKDGNLETRLPPLGSQELDQLASGINRMAETLQ
NAQEELQHSIDQATEDVRQNLETIEIQNIELDLARKEALEASRIKSEFLANMSHEIRTPL
NGILGFTHLLQKSELTPRQLDYLGTIEKSADNLLGIINEILDFSKIEAGKLVLDSIPFNL
RDLLQDTLTILAPAAHAKQLELVSLVYRDTPLALVGDPLRLKQILTNLISNAIKFTREGT
IVARAMIEDEQEDSVQLRISVQDTGIGLSSQDVRALFQAFSQADNSLSRQPGGTGLGLVI
SKRLIEQMGGEIGVDSTPGEGSEFWISLNLPKTRDDVEDLPSAPLLGHRVAVLENHELAR
QALQHQLEDCGLEVTPFNSLESLTNGITSAHQTEQAIDLAVLGVTANDIPPERLNQHLWD
LEHLGCKVLVLCPTTEQMLFNQSVPNPNSQLQAKPACTRKLRRALSDLISPRPSRSEPGE
PLSSRAPRVLCVDDNPANLLLVQTLLEDMGARVRAVESGYAAIDAVKQDTFDLVLMDVQM
PGMDGRQSTEAIRQWESERSGTPLPVVALTAHAMANEKRALLQSGMDDYLTKPISERQLA
QVVLKWTGLALRNQGPERVADGLGPGVQLPVLDHEEGLRLAAGKADLAADMLAMLLASLE
ADRLAITIARDANDNNALIERVHRLHGATRYCGVPQLRAACHRAETLLKQDDAKAQHALD
ELDMAIARLASEARVNA