Protein Info for GFF3182 in Variovorax sp. SCN45

Annotation: Cyclopropane-fatty-acyl-phospholipid synthase-like protein, clusters with FIG005069

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 348 PF02353: CMAS" amino acids 67 to 304 (238 residues), 141.9 bits, see alignment E=1.1e-44 PF13489: Methyltransf_23" amino acids 109 to 249 (141 residues), 53.1 bits, see alignment E=1.2e-17 PF13847: Methyltransf_31" amino acids 121 to 232 (112 residues), 48.4 bits, see alignment E=3.5e-16 PF05175: MTS" amino acids 121 to 223 (103 residues), 30.4 bits, see alignment E=1.1e-10 PF03848: TehB" amino acids 123 to 234 (112 residues), 29.2 bits, see alignment E=2.4e-10 PF13649: Methyltransf_25" amino acids 125 to 219 (95 residues), 66.6 bits, see alignment E=1.1e-21 PF08242: Methyltransf_12" amino acids 126 to 221 (96 residues), 54.5 bits, see alignment E=6.5e-18 PF08241: Methyltransf_11" amino acids 126 to 222 (97 residues), 61.4 bits, see alignment E=4.2e-20

Best Hits

Swiss-Prot: 31% identical to PNMT_THLFG: Pavine N-methyltransferase from Thalictrum flavum subsp. glaucum

KEGG orthology group: K00574, cyclopropane-fatty-acyl-phospholipid synthase [EC: 2.1.1.79] (inferred from 87% identity to vpe:Varpa_4424)

MetaCyc: 45% identical to phenylalkylamine N-methyltransferase (Ephedra sinica)
2.1.1.M76 [EC: 2.1.1.M76]; 2.1.1.M76 [EC: 2.1.1.M76]; 2.1.1.M76 [EC: 2.1.1.M76]; 2.1.1.M76 [EC: 2.1.1.M76]; 2.1.1.M76 [EC: 2.1.1.M76]

Predicted SEED Role

"Cyclopropane-fatty-acyl-phospholipid synthase-like protein, clusters with FIG005069"

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.79

Use Curated BLAST to search for 2.1.1.79 or 2.1.1.M76

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (348 amino acids)

>GFF3182 Cyclopropane-fatty-acyl-phospholipid synthase-like protein, clusters with FIG005069 (Variovorax sp. SCN45)
MPQDIAFAIRWVEKGLVPDPVVRRGIRRLLKDRLAELHSGNALAVAELTQDFVARMGAAN
LAPLPEKANEQHYEVPAAFFGEVLGNHRKYSSCYWPEGTRTLEEAESAALAATCERAGLA
DGQDVLELGCGWGSLTLWMAEHYPGSRVTALSNSNSQREYIEAEAARRGLGNVRVLTRDI
NVFDTDERFDRVVSVEMFEHLRNWPQAFANVARWLRPGGRFFMHVFAHKEAPYPFEVRDP
SDWMSKYFFSGGMMPSDDLALHCQDDLRLLRRWRWEGSHYQRTAEAWLRNMDERRDTLRP
LFQATYGAEANVWWTRWRLFFMSVAELFGFDHGQRWWVSHYLFERRGA