Protein Info for GFF3167 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868

Annotation: Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 947 PF03710: GlnE" amino acids 31 to 273 (243 residues), 312 bits, see alignment E=2.9e-97 amino acids 554 to 806 (253 residues), 355.7 bits, see alignment E=1.4e-110 PF08335: GlnD_UR_UTase" amino acids 297 to 436 (140 residues), 109 bits, see alignment E=2e-35 amino acids 826 to 918 (93 residues), 38.6 bits, see alignment E=1e-13

Best Hits

Swiss-Prot: 100% identical to GLNE_SALCH: Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme (glnE) from Salmonella choleraesuis (strain SC-B67)

KEGG orthology group: K00982, glutamate-ammonia-ligase adenylyltransferase [EC: 2.7.7.42] (inferred from 89% identity to cko:CKO_04439)

MetaCyc: 88% identical to fused glutamine synthetase deadenylase/glutamine synthetase adenylyltransferase (Escherichia coli K-12 substr. MG1655)
GSDEADENYLATION-RXN [EC: 2.7.7.89]; [Glutamate--ammonia-ligase] adenylyltransferase. [EC: 2.7.7.89, 2.7.7.42]

Predicted SEED Role

"Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42)" in subsystem Ammonia assimilation (EC 2.7.7.42)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.42 or 2.7.7.89

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (947 amino acids)

>GFF3167 Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42) (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868)
MTPLSSPLSQYWQTVVERLPEGFTETSLSVQAKSVLTFSDFALDSVIAHPEWLAELESAS
PQADEWRHYAGWLQEALAGVCDDASLMRELRFFRRRIMVRIAWAQTLSLVDDETILQQLS
HLAETLIVGARDWLYAACCREWGTPCNPQGVPQPLLILGMGKLGGGELNFSSDIDLIFAW
PEHGETRGGRRELDNAQFFTRLGQRLIKALDQPTMDGFVYRVDMRLRPFGDSGPLVLSFA
ALEDYYQEQGRDWERYAMVKARLMGDNDDAWSRELRAMLRPFVFRRYIDFSVIQSLRNMK
GMIAREVRRRGLKDNIKLGAGGIREIEFIVQVFQLIRGGREPSLQSRSLLPTLDAIAALH
LLPENDVAQLRVAYLFLRRLENLLQSINDEQTQTLPADDLNRARLAWGMKAENWPQLVGE
LTDHMANVRRVFNELIGDDEADTPQEEERSEPWREVWQDALQEDDSTPVLAHLADEERRQ
VLTLIADFRKELDKRPIGPRGRQVLDQLMPHLLADVCSREDAAVTLSRITPLLAGIVTRT
TYLELLSEFPGALKHLIMLCAASPMIASQLARYPLLLDELLDPGTLYQPTATDAYRDELR
QYLLRVPEEDEEQQLEALRQFKQAQLLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQ
QAWTQMVARYGQPAHLDERQGRGFAVVGYGKLGGWELGYSSDLDLIFLHDCPMDVMTNGE
REIDGRQFYLRLAQRIMHLFSTRTSSGILYEVDARLRPSGAAGMLVTSADAFADYQQHEA
WTWEHQALVRARVVYGDPQLTSQFDAVRRTIMTTARDGKTLQTEVREMREKMRAHLGNKH
RDRFDIKADEGGITDIEFIAQYLVLRYAHEKPKLTRWSDNVRILELLAQNGIMDEHEAQA
LTVAYTTLRDELHHLALQELPGHVAQTCFSKERALVQASWRKWLVAV