Protein Info for GFF3158 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868

Annotation: Periplasmic thiol:disulfide interchange protein, DsbA-like

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 223 signal peptide" amino acids 1 to 30 (30 residues), see Phobius details PF13462: Thioredoxin_4" amino acids 42 to 209 (168 residues), 32.5 bits, see alignment E=9.8e-12 PF01323: DSBA" amino acids 48 to 202 (155 residues), 44.2 bits, see alignment E=2e-15

Best Hits

Swiss-Prot: 90% identical to DSBL_LELAM: Thiol:disulfide interchange protein DsbL (dsbL) from Lelliottia amnigena

KEGG orthology group: K03673, thiol:disulfide interchange protein DsbA (inferred from 100% identity to sew:SeSA_A3379)

Predicted SEED Role

"Periplasmic thiol:disulfide interchange protein, DsbA-like" in subsystem Periplasmic disulfide interchange

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (223 amino acids)

>GFF3158 Periplasmic thiol:disulfide interchange protein, DsbA-like (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868)
MSSKWITSLFKSVVLTAALVTPFAASAFTEGTDYMVLEKPIPNADKTLIKVFSYACPFCY
KYDKAVTGPVSDKVADLVTFTPFHLETKGEYGKQASEVFAVLIAKDKAAGISLFDAKSQF
KKAKFAWYAAYHDKKERWSDGKDPAAFIKTGLDAAGMSQADFEAALKDPAVQETLEKWKA
AYDVAKIQGVPAYVVNGKYLIYTKNIKSIDSMAELVRELATKK