Protein Info for GFF315 in Variovorax sp. SCN45

Annotation: FIGfam138462: Acyl-CoA synthetase, AMP-(fatty) acid ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 570 PF00501: AMP-binding" amino acids 14 to 303 (290 residues), 52.5 bits, see alignment E=5.3e-18 PF13193: AMP-binding_C" amino acids 379 to 445 (67 residues), 26.5 bits, see alignment E=1.5e-09 PF07977: FabA" amino acids 471 to 541 (71 residues), 27.6 bits, see alignment E=3.1e-10

Best Hits

KEGG orthology group: None (inferred from 90% identity to vpe:Varpa_3237)

Predicted SEED Role

"FIGfam138462: Acyl-CoA synthetase, AMP-(fatty) acid ligase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (570 amino acids)

>GFF315 FIGfam138462: Acyl-CoA synthetase, AMP-(fatty) acid ligase (Variovorax sp. SCN45)
VTTTDNFLLPLLADRDPDAPLAWRAGVPVSTRQFLADVARFAPELPEDGPAVNLCVDRYA
FAVSLGAALVRGHASLLPPDARPDTLARLVESGGPRLFALTDDPKLQTPGMRRVLIEDRS
SLEGDASGAVPQINAGMHAVSLLTSGSTGVPQPHAKTWGTLVGDVAVAVKRLCRVLGRSS
LEGLTLVATVPVQHSYGIESSALLAMLGGAAFESGRPFFPADVAQTLASVPRPRALVTTP
FHLKTLLLSGIALPPVDLILSATAPLSPQLALQAEQAMGAVLLEIYGSTESGQVATRRTT
ESEVWETFGDIRVHAEPGEGGGPERFIFSGDFIPEPTPMADVLELLDERRFRLFGRANDL
IHVAGRRSSLAHLNYHLNSIPGVEDGAFWLPDEVADGVVRPVAFVVAPTLSAGEIIAALR
QRLEAVFVPRRVVQVKAFPREGTGKLTVRALREFALAQLAEDDTPVQLAHTVPVDHPVFA
GHFPGQPLVPGALLLAEVMEAVQRVPALANRLGANPTLAAAKFLAPVRPGSTLSIELHPE
AGAGRGVRFDVRCDGVVAVSGRWTAVQESA