Protein Info for GFF3147 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: IncF plasmid conjugative transfer pilus assembly protein TraB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 472 transmembrane" amino acids 32 to 52 (21 residues), see Phobius details PF03743: TrbI" amino acids 245 to 439 (195 residues), 120.6 bits, see alignment E=3.7e-39

Best Hits

KEGG orthology group: K12065, conjugal transfer pilus assembly protein TraB (inferred from 83% identity to del:DelCs14_1694)

Predicted SEED Role

"IncF plasmid conjugative transfer pilus assembly protein TraB" in subsystem Type 4 secretion and conjugative transfer

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (472 amino acids)

>GFF3147 IncF plasmid conjugative transfer pilus assembly protein TraB (Hydrogenophaga sp. GW460-11-11-14-LB1)
MSTPSTPPGAARPLGHAVRGMLDRLSPRQRQYALLAAILGGGIGLLWLIFSFSAPNPQPH
GGRTVQAQPGDVTNIGVLPPGGQVNPVDQWVGTAGRKLAQYETEREEQGRLNKDRQAFES
RTMQRFAELEQRLTANQQAPTPAAPVPPPPSRPAPASPAAMPPAAGLPPAQPPSGGGAMP
VGPPTFQPTVPPAPPAPPVPVISRVTLADRSRPSAAASHDGSARTEGTASPSNAPKTVST
FLPVSFTRGILLGGLDAPTGGQSQSNPHPVLIRLSDNSVLPNQFRGEYRECFVVAAGYGD
ISSERAYLRTENLSCVRADGATLEVRIQGSVYGEDGKVGMRGRLVTKQGQMLANALLAGV
VSGIGQGLATANTTYSSSPLGTVASTDSNADAYRAGLGSGVGKALDRLAQYYIKLAENTF
PVIEVDAGRQIDVVITKGVRIDVPMTALAERGSRRVASPDTRYLEAPDDGNY