Protein Info for GFF3127 in Sphingobium sp. HT1-2

Annotation: Ribonuclease D (EC 3.1.26.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 388 TIGR01388: ribonuclease D" amino acids 6 to 370 (365 residues), 368.6 bits, see alignment E=2e-114 PF01612: DNA_pol_A_exo1" amino acids 7 to 173 (167 residues), 135.4 bits, see alignment E=1.9e-43 PF00570: HRDC" amino acids 216 to 282 (67 residues), 52.3 bits, see alignment E=4.4e-18

Best Hits

Swiss-Prot: 90% identical to RND_SPHJU: Ribonuclease D (rnd) from Sphingobium japonicum (strain DSM 16413 / CCM 7287 / MTCC 6362 / UT26 / NBRC 101211 / UT26S)

KEGG orthology group: K03684, ribonuclease D [EC: 3.1.13.5] (inferred from 90% identity to sjp:SJA_C1-32920)

Predicted SEED Role

"Ribonuclease D (EC 3.1.26.3)" in subsystem Experimental tye or tRNA processing (EC 3.1.26.3)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 3.1.13.5, 3.1.26.3

Use Curated BLAST to search for 3.1.13.5 or 3.1.26.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (388 amino acids)

>GFF3127 Ribonuclease D (EC 3.1.26.3) (Sphingobium sp. HT1-2)
MQIHPLITDSKTLSDFCARMAKSPYVAVDTEFMRENSYWPELCLVQVADSSEAAAIDPKA
PGIDLTPLLDLLVNNEDVLKVFHAGGQDIEIVHNLTGKTPHPMFDTQIAAMALGLGEQIG
YGNLVDAWLGVQLDKGARFTDWARRPLDKRQIDYAIGDVTYLIEIFPKMLEELRKTGRGD
WLDQEMERISDPSNYENDPSEAWKRVRIASRKADVLGRLKALAAWREKEAQDKNLPRGRI
VKDETLADIASHPPRAQEDLGKVRGLSATWKSNDIGGRLMAALGAHKPLGKDEMPERDPK
RPGLGKDGALVADLLKLLLKIRSRDINVAPRLLARTDDIEALAAGVRDDLAILEGWRYDQ
FGRDAVDLVEGRLAFAVKNGRLKMTRTE