Protein Info for GFF3125 in Sphingobium sp. HT1-2

Annotation: Aspartyl-tRNA synthetase (EC 6.1.1.12) @ Aspartyl-tRNA(Asn) synthetase (EC 6.1.1.23)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 594 TIGR00459: aspartate--tRNA ligase" amino acids 5 to 588 (584 residues), 609.6 bits, see alignment E=2.7e-187 PF01336: tRNA_anti-codon" amino acids 21 to 104 (84 residues), 52.7 bits, see alignment E=4.9e-18 PF00152: tRNA-synt_2" amino acids 122 to 562 (441 residues), 365.1 bits, see alignment E=4e-113 PF02938: GAD" amino acids 316 to 407 (92 residues), 47.9 bits, see alignment E=2e-16

Best Hits

Swiss-Prot: 80% identical to SYDND_NOVAD: Aspartate--tRNA(Asp/Asn) ligase (aspS) from Novosphingobium aromaticivorans (strain ATCC 700278 / DSM 12444 / CIP 105152 / NBRC 16084 / F199)

KEGG orthology group: K01876, aspartyl-tRNA synthetase [EC: 6.1.1.12] (inferred from 89% identity to sjp:SJA_C1-32910)

Predicted SEED Role

"Aspartyl-tRNA synthetase (EC 6.1.1.12) @ Aspartyl-tRNA(Asn) synthetase (EC 6.1.1.23)" (EC 6.1.1.12, EC 6.1.1.23)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.12 or 6.1.1.23

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (594 amino acids)

>GFF3125 Aspartyl-tRNA synthetase (EC 6.1.1.12) @ Aspartyl-tRNA(Asn) synthetase (EC 6.1.1.23) (Sphingobium sp. HT1-2)
MHAYRTHNCGALREADVGANVRLSGWVHRKRDHGGVLFIDLRDHYGLTQIVAKADSEPLR
ILDGLRAESVVTIDGTVVARGPEATNPKMATGTIEVVADKVTVLSTAAELPLPVAGEQEY
PEDIRLRYRFLDLRRETLHANIVTRTKIIRDMRRRMEDAGFTEYSTPILTASSPEGARDF
LVPSRIHAGKFYALPQAPQQYKQLLMVAGFDRYFQIAPCFRDEDPRADRLPGEFYQLDLE
MSFVTQEDVWNTMEPVIASVFEEFAQGKPVTPAGSFPRIPHAEAMLKYGSDKPDLRNPII
IQDVTEHFHGSGFGIFASLVESGNVIRAIPAPGAGAGSRKFFDDMNNWARGEGYSGLGYI
NIKDGVPGGPIAKNHGEEATAKLIAALGLGPNDGVFFAAGKESQAAKLAGLARIRVGETL
DLIDKDRFDLCWIVDFPFYEYDEDEKKVDFAHNPFSMPQGGLDALNNQDPLSLKAYQYDM
VCNGYEIASGSIRNQSPEAMVKAFELVGLSQADVEERFGGLYRAFQYGAPPHGGMAAGVD
RIVMLLCGAQNLREITLFPMNQRAEDLLMGAPSAAEFKQLRELHVRVVEPQAKA