Protein Info for GFF3105 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868

Annotation: Uptake hydrogenase large subunit (EC 1.12.99.6)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 567 PF27537: HoxH" amino acids 4 to 26 (23 residues), 28.8 bits, see alignment (E = 1.1e-10) PF00374: NiFeSe_Hases" amino acids 39 to 552 (514 residues), 711.6 bits, see alignment E=6.8e-218

Best Hits

Swiss-Prot: 94% identical to MBHM_ECO57: Hydrogenase-2 large chain (hybC) from Escherichia coli O157:H7

KEGG orthology group: K06281, hydrogenase large subunit [EC: 1.12.99.6] (inferred from 94% identity to eco:b2994)

MetaCyc: 94% identical to hydrogenase 2 large subunit (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Uptake hydrogenase large subunit (EC 1.12.99.6)" in subsystem Hydrogenases or Membrane-bound Ni, Fe-hydrogenase (EC 1.12.99.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.12.99.6

Use Curated BLAST to search for 1.12.99.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (567 amino acids)

>GFF3105 Uptake hydrogenase large subunit (EC 1.12.99.6) (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868)
MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEIVKNRDPRDAWMIVQRI
CGVCTTTHAISSVRAAESALNIDVPVNAQYIRNIILAAHTTHDHIVHFYQLSALDWVDIT
SALKADPEKASAMLDGVSSWHLNSAQEFTKVQNKIKDLVASGQLGIFANGYWGHPAMQLP
PEVNLIAVAHYLQALECQRDANRVVALLGGKSPHIQNLAVGGVANPINLDSIGTLNLERL
MYIKSFIDKLSDFVEQVYKVDTAVIAAYYPEWLERGKGAVNYLSAPEFPTDGKNGSFLFP
GGYITNADLSTYRPITSHSDEFLIKGIQESAKHAWYKDEAPQAPWEGTTIPAYDGWSDDG
KYSWVKSPTFYGKTVEVGPLANMLCKLAAGRESTQTKLNEIIALYQKLTGKTLEMAQLHS
TLGRIIGRTVHCCELQNILQDQYNALIVNIGKGDYTTFVKPNIPATGEFKGVGFLEAPRG
MLSHWMVIKDGIISNYQAVVPSTWNSGPRNFNDDVGPYEQSLVGTPIADPAKPLEVVRTI
HSFDPCMACAVHVVDADGNEVVSVKVL