Protein Info for GFF3056 in Variovorax sp. SCN45

Annotation: Nitrogen assimilation regulatory protein Nac

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 323 PF00126: HTH_1" amino acids 14 to 73 (60 residues), 70.6 bits, see alignment E=8.9e-24 PF03466: LysR_substrate" amino acids 98 to 302 (205 residues), 121.9 bits, see alignment E=2.5e-39

Best Hits

Swiss-Prot: 57% identical to NAC_KLEAE: Nitrogen assimilation regulatory protein nac (nac) from Klebsiella aerogenes

KEGG orthology group: None (inferred from 97% identity to vap:Vapar_6361)

Predicted SEED Role

"Nitrogen assimilation regulatory protein Nac" in subsystem DNA-binding regulatory proteins, strays

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (323 amino acids)

>GFF3056 Nitrogen assimilation regulatory protein Nac (Variovorax sp. SCN45)
MLGLAFAWGQGVNLRRLKYFVKIVDIGSLTQAAEVLFIAQPALSQQLATLEGEVRQQLLV
RTKRGVTPTEAGKVLYRHAQIILRQCEQARVDMEAAGEGLSGQVSVGLAPGTAASALSLP
LLRTVRARHPGILLYLNENYGTTLSELIMNGRMDLAVLYGDKAIHGLTFLPLLKEPLFLV
GPASMPAPSQPVKLADLRDIELFLPRPYNVVRKLVDAAFVRAGMVPRVVAEIESAFTLTA
AIADGLGATILPASMAREVVATCGAWQFPIVDPVIEAPLALCQSDHLPLSEPAQAVKDIL
LELVVDLAGTVASTPEPELSALS