Protein Info for GFF3054 in Variovorax sp. SCN45

Annotation: Biotin carboxylase of acetyl-CoA carboxylase (EC 6.3.4.14)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00289: Biotin_carb_N
  • amino acids 1 to 110 (110 residues), 142.1 bits, E=2.4e-45
• TIGR00514: acetyl-CoA carboxylase, biotin carboxylase subunit
  • amino acids 1 to 445 (445 residues), 671.7 bits, E=2.5e-206
• PF02786: CPSase_L_D2
  • amino acids 115 to 322 (208 residues), 263 bits, E=4.7e-82
• PF02222: ATP-grasp
  • amino acids 145 to 292 (148 residues), 32.7 bits, E=1.4e-11
• PF07478: Dala_Dala_lig_C
  • amino acids 147 to 291 (145 residues), 25.1 bits, E=2.8e-09
• PF02785: Biotin_carb_C
  • amino acids 336 to 441 (106 residues), 132.6 bits, E=1.5e-42

Best Hits

Swiss-Prot: 64% identical to ACCC_PSEAE: Biotin carboxylase (accC) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K01961, acetyl-CoA carboxylase, biotin carboxylase subunit [EC: 6.3.4.14 6.4.1.2] (inferred from 92% identity to vap:Vapar_6363)

MetaCyc: 63% identical to biotin carboxylase (Escherichia coli K-12 substr. MG1655)
Biotin carboxylase. [EC: 6.3.4.14]

Predicted SEED Role

"Biotin carboxylase of acetyl-CoA carboxylase (EC 6.3.4.14)" in subsystem Fatty Acid Biosynthesis FASII (EC 6.3.4.14)

MetaCyc Pathways

• biotin-carboxyl carrier protein assembly (4/4 steps found)
• 3-hydroxypropanoate cycle (9/13 steps found)
• fatty acid biosynthesis initiation (type I) (1/3 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (11/18 steps found)
• superpathway of the 3-hydroxypropanoate cycle (11/18 steps found)
• glyoxylate assimilation (7/13 steps found)
• jadomycin biosynthesis (3/9 steps found)
• superpathway of mycolate biosynthesis (27/239 steps found)

KEGG Metabolic Maps

• Fatty acid biosynthesis
• Propanoate metabolism
• Pyruvate metabolism
• Tetracycline biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 6.3.4.14, 6.4.1.2

Use Curated BLAST to search for 6.3.4.14 or 6.4.1.2

Protein Sequence (450 amino acids)

>GFF3054 Biotin carboxylase of acetyl-CoA carboxylase (EC 6.3.4.14) (Variovorax sp. SCN45)
MFDTVLIANRGEIALRIQRACRGLGLRTVAVHSEADRDAVYVAQADQALCIGPAAPGLSY
LNQSAILFAAEVSGAQAIHPGYGFLSENAGFAARCEEAGLAFVGPSAACIGTMGDKVSAK
RAMRKAGVPCVPGPDEPLPDDPAAVLALARDIGYPVIVKAAGGGGGRGMRVVHEEAALLD
AMALTREEARQAFGNPEVYIEKFLLHPRHVEIQVLADSHGNAVWLGSRDCSLQRRHQKVI
EEAPAPGIDDALMAEVGERCAAACRQIGYCGVGTFEFLYEKGAFYFIEMNTRLQVEHPVT
EMTTGIDIVQQQLRMARGERLTIAQADVRCQGHAVECRINAENPDTFAPAPGRITGWQVP
GGFGVRVDSHAGAGYRVPPYYDSMIAKLIVHGSTRADALERMRLALAETRVDGIATNVPL
HRALLQDEGFAAGGVDIHHLERWLQQRSAA