Protein Info for PS417_15565 in Pseudomonas simiae WCS417

Annotation: transketolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00456: Transketolase_N
  • amino acids 9 to 355 (347 residues), 419.9 bits, E=1.3e-129
• TIGR00232: transketolase
  • amino acids 11 to 681 (671 residues), 811.5 bits, E=2.7e-248
• PF02779: Transket_pyr
  • amino acids 371 to 543 (173 residues), 156 bits, E=1.6e-49
• PF22613: Transketolase_C_1
  • amino acids 559 to 670 (112 residues), 137.1 bits, E=5.9e-44
• PF02780: Transketolase_C
  • amino acids 566 to 636 (71 residues), 33.6 bits, E=6.6e-12

Best Hits

Swiss-Prot: 54% identical to TKT_GEOSE: Transketolase (tkt) from Geobacillus stearothermophilus

KEGG orthology group: K00615, transketolase [EC: 2.2.1.1] (inferred from 93% identity to pfs:PFLU3599)

Predicted SEED Role

"Transketolase (EC 2.2.1.1)" in subsystem Calvin-Benson cycle or Pentose phosphate pathway (EC 2.2.1.1)

MetaCyc Pathways

• superpathway of glucose and xylose degradation (16/17 steps found)
• formaldehyde assimilation III (dihydroxyacetone cycle) (11/12 steps found)
• pentose phosphate pathway (8/8 steps found)
• pentose phosphate pathway (non-oxidative branch) I (5/5 steps found)
• Bifidobacterium shunt (12/15 steps found)
• pentose phosphate pathway (partial) (3/3 steps found)
• Rubisco shunt (8/10 steps found)
• pentose phosphate pathway (non-oxidative branch) II (5/6 steps found)
• Calvin-Benson-Bassham cycle (10/13 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (19/26 steps found)
• ethene biosynthesis V (engineered) (18/25 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (19/27 steps found)
• formaldehyde assimilation II (assimilatory RuMP Cycle) (6/9 steps found)
• oxygenic photosynthesis (11/17 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of ansamycins
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Carbon fixation in photosynthetic organisms
• Pentose phosphate pathway

Isozymes

Compare fitness of predicted isozymes for: 2.2.1.1

Use Curated BLAST to search for 2.2.1.1

Sequence Analysis Tools

See A0A1N7UAP7 at UniProt or InterPro

Protein Sequence (685 amino acids)

>PS417_15565 transketolase (Pseudomonas simiae WCS417)
MTHAATDIDTQCINTLRTLAMDAVQKANSGHPGTPMGLAPVGYTLWSRFLRYHPQHPDWP
SRDRFVLSVGHASMLLYSLLHLAGVVELDANGKRTGQPAVSLDDIKQFRQAGSKTPGHPE
YRMTTGVETTTGPLGQGCANSVGMAMAERWLAKRFNREEKTLFDYNVYTLCGDGDMMEGI
SGEAASLAGHLKLDKLCWIYDNNTISIEGHTELAFSEDVIQRFQAYGWHTLHVTDANDLQ
ALSNALEAFHCNRGAPTLIVVDSVIGYGSPHKHNTAAAHGEPLGEEEIRLTKSAYGWPED
SSFLVPEAARAQLRDALLERSEPLYEKWTEHLSLLEQFEPGLADELRRMRAGEMPEHWQK
HLPDFDTDPKGIASRAAGGEVLNAFAEQVPWLLGGSADLSPSTKTNLTFDGAGRFSADDY
SGRNLHFGIREHAMGAIANGLALSYLRPYTSTFLVFSDYMKPPIRLAAIMELPVVFVFTH
DSIGVGEDGPTHQPIEHLTQLRATPGLLTLRPGDANETLELWKVALAQTHRPSCVVLSRQ
PLPTLDRTKYAAASGAAKGAYVLAAADHPKVLLLATGSEVGLAVAAYEQLKTEGIAAQVV
SMPSWELFEDQDQAYRDSVLPPAVKARVVVEQAGPLGWDRYVGQTGAKVVMNSFGASAPL
AKLQEKFGFTVENVVRQAKQQIQQT