Protein Info for GFF301 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868

Annotation: Ribokinase (EC 2.7.1.15)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 306 PF00294: PfkB" amino acids 2 to 293 (292 residues), 221.9 bits, see alignment E=1.2e-69 TIGR02152: ribokinase" amino acids 3 to 299 (297 residues), 374.7 bits, see alignment E=1.7e-116 PF08543: Phos_pyr_kin" amino acids 176 to 280 (105 residues), 39.9 bits, see alignment E=3.4e-14

Best Hits

Swiss-Prot: 39% identical to RBSK_MOUSE: Ribokinase (Rbks) from Mus musculus

KEGG orthology group: K00852, ribokinase [EC: 2.7.1.15] (inferred from 100% identity to sec:SC3713)

MetaCyc: 100% identical to deoxyribokinase monomer (Salmonella enterica enterica serovar Typhi)
RXN-14223 [EC: 2.7.1.229]

Predicted SEED Role

"Ribokinase (EC 2.7.1.15)" in subsystem D-ribose utilization or Deoxyribose and Deoxynucleoside Catabolism (EC 2.7.1.15)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.15

Use Curated BLAST to search for 2.7.1.15 or 2.7.1.229

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (306 amino acids)

>GFF301 Ribokinase (EC 2.7.1.15) (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868)
MDIAVIGSNMVDLITYTNQMPKEGETLEAPAFKIGCGGKGANQAVAAAKLNSKVLMLTKV
GDDIFADNTIRNLESWGINTTYVEKVPCTSSGVAPIFVNANSSNSILIIKGANKFLSPED
IDRAAEDLKKCQLIVLQLEVQLETVYHAIEFGKKHGIEVLLNPAPALRELDMSYACKCDF
FVPNETELEILTGMPVDTYDHIRAAARSLVDKGLNNIIVTMGEKGALWMTRDQEVHVPAF
RVNAVDTSGAGDAFIGCFAHYYVQSGDVEAAMKKAVLFAAFSVTGKGTQSSYPSIEQFNE
YLSLNE