Protein Info for GFF3 in Xanthobacter sp. DMC5

Annotation: Transcription termination/antitermination protein NusG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 176 PF02357: NusG" amino acids 3 to 106 (104 residues), 103.9 bits, see alignment E=5.6e-34 TIGR00922: transcription termination/antitermination factor NusG" amino acids 5 to 176 (172 residues), 229 bits, see alignment E=1.6e-72 PF00467: KOW" amino acids 125 to 153 (29 residues), 28 bits, see alignment 1.4e-10

Best Hits

Swiss-Prot: 58% identical to NUSG_NEIMB: Transcription termination/antitermination protein NusG (nusG) from Neisseria meningitidis serogroup B (strain MC58)

KEGG orthology group: K02601, transcriptional antiterminator NusG (inferred from 96% identity to xau:Xaut_3359)

Predicted SEED Role

"Transcription antitermination protein NusG" in subsystem Transcription factors bacterial

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (176 amino acids)

>GFF3 Transcription termination/antitermination protein NusG (Xanthobacter sp. DMC5)
MAKRWYIVHAYSNFEKKVADSIREQADQRGLTDLFEQILVPTEKVVEVRRGRKVDTERKF
FPGYVLVKMDLTDEAFHLIKNTPKVTGFLGADNKPMPISESEAMRILQQVQEGIERPKPS
ISYEVGETVKVSDGPFASFNGIVEEVDDSRSRLKVAVSIFGRATPVELEFGQVEKV