Protein Info for PS417_15330 in Pseudomonas simiae WCS417

Annotation: type VI secretion protein ImpA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 517 TIGR03362: type VI secretion-associated protein, VC_A0119 family" amino acids 14 to 483 (470 residues), 381.8 bits, see alignment E=3e-118 PF06812: ImpA_N" amino acids 18 to 127 (110 residues), 91.6 bits, see alignment E=4e-30 PF16989: T6SS_VasJ" amino acids 228 to 482 (255 residues), 237.4 bits, see alignment E=1.9e-74

Best Hits

KEGG orthology group: K11910, type VI secretion system protein VasJ (inferred from 82% identity to pfs:PFLU3549)

Predicted SEED Role

"Uncharacterized protein ImpA"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7U2I6 at UniProt or InterPro

Protein Sequence (517 amino acids)

>PS417_15330 type VI secretion protein ImpA (Pseudomonas simiae WCS417)
MSYSDTLCDYYLEVARSPCSQTCFAGGDMRFSSEYECLESELTKAQSIHGTRQPDWHKIL
ETSERLLRQQSKDLRVVVWMTWALYQRESYPGLLAGLGLLRYLCEHHWVESYPEKLRTRS
AAFAWLVLRIEPLFAQGLPLQNQQLLFRAVLEHLTSLEELWGRHLGDDAPLLLPIRRQLA
QRLEQALQADAPAAGLSGVIAQVKQATSQLLKSEPVVDNEKDAGKLLRTLQEQARPLCAW
WLRQNATDLRALRLNRTLNWLVLSGYPDADSERVTTLRGPAPEKLKRYQERFAQGHHADL
VLELEASLAGAMFWFDGLHMLWQCLEALRAEQAMIELEVTFALLLQRLPDLPSFCFHDGI
PFADAASRDWITLQVSRHLQTPESAAVVIDTEAEPWESALQAVAPRLRQDGLKAAIHELK
QGMYAARSERARFHWRLAQARLCVQAGKHELAKIQLEHLDHELQHTGLEHWEPELALQVT
QLLYRCCDLLPQNHAVRERKEDTHRRLCLFDLEAVLE