Protein Info for GFF2986 in Pseudomonas sp. DMC3

Annotation: Alkaline phosphatase L

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 367 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF12849: PBP_like_2" amino acids 45 to 311 (267 residues), 71.5 bits, see alignment E=5.2e-24

Best Hits

Swiss-Prot: 67% identical to PPBL_PSEAE: Alkaline phosphatase L (phoA2) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: None (inferred from 84% identity to pfo:Pfl01_3172)

Predicted SEED Role

"Phosphate-binding DING protein (related to PstS)"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (367 amino acids)

>GFF2986 Alkaline phosphatase L (Pseudomonas sp. DMC3)
MFKRTLIAASLTVAALASAQAMAVTGGGASLPAALYKGSANSILPANFSYAVTGSGIGKS
AFLTNNAAQFSTTGTVHFAASDSILSATELSTYTSNFGASYGPLIQLPSVATSVAIPYKK
SGQTALNLTSAQLCDALSGTKTTWGALLGTADTTAIRVVYRSTSSGTTEILSRHLNSICP
TKFAVNSTFTNARLPAGSALPSTWVGVANTADVATAVNAVDGSIGYVGPDGVNATSNAVV
ARVNGVQPTNANVTLALSTAPVPSAPANPAGWAPVVPNPASGYPIVAYTNFIFGQCYKDA
AVATAVRNFLTTHYSNPGNNVATVAHSFTPVPNNWKTAVTANFITNTSGNNLDINNANAC
NGIGRPL