Protein Info for GFF2984 in Xanthobacter sp. DMC5

Annotation: Potassium-transporting ATPase ATP-binding subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 687 transmembrane" amino acids 34 to 52 (19 residues), see Phobius details amino acids 64 to 82 (19 residues), see Phobius details amino acids 218 to 242 (25 residues), see Phobius details amino acids 248 to 274 (27 residues), see Phobius details amino acids 590 to 609 (20 residues), see Phobius details amino acids 621 to 640 (20 residues), see Phobius details amino acids 660 to 684 (25 residues), see Phobius details TIGR01497: K+-transporting ATPase, B subunit" amino acids 6 to 682 (677 residues), 1052.4 bits, see alignment E=0 TIGR01494: HAD ATPase, P-type, family IC" amino acids 72 to 324 (253 residues), 115.2 bits, see alignment E=2.7e-37 amino acids 371 to 608 (238 residues), 147.6 bits, see alignment E=4.1e-47 PF00122: E1-E2_ATPase" amino acids 103 to 282 (180 residues), 118.2 bits, see alignment E=3.1e-38 PF00702: Hydrolase" amino acids 299 to 535 (237 residues), 91.3 bits, see alignment E=1.1e-29

Best Hits

Swiss-Prot: 82% identical to KDPB_RHILO: Potassium-transporting ATPase ATP-binding subunit (kdpB) from Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099)

KEGG orthology group: K01547, K+-transporting ATPase ATPase B chain [EC: 3.6.3.12] (inferred from 89% identity to xau:Xaut_3238)

Predicted SEED Role

"Potassium-transporting ATPase B chain (EC 3.6.3.12) (TC 3.A.3.7.1)" in subsystem Potassium homeostasis (EC 3.6.3.12, TC 3.A.3.7.1)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.12

Use Curated BLAST to search for 3.6.3.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (687 amino acids)

>GFF2984 Potassium-transporting ATPase ATP-binding subunit (Xanthobacter sp. DMC5)
MAKPRSSSILDPAILVPAIGASFKKLDPRAMVKNPVMFVVEVVALLTTVLFIRDLATGGS
GLGFSFQIILWLWFTVLFANFAEAVAEGRGKAQADSLRRTRTETQAKLLTGSGDDWREVP
GTSLKVGDVVLVEAGDVIPSDGEVVQGVASVNEAAITGESAPVIRESGGDRSAVTGGTTV
ISDHIKVRITAAAGSTFLDRMIHLVEGAERQKTPNEIALNILLAGMTLIFVFATASIPSF
AAYAGGTISVVVLVALFVTLIPTTIGALLSAIGIAGMDRLVRFNVLAMSGRAVEAAGDVD
TLLLDKTGTITLGNRQAAEFRPVRGVSPQELADAAQLASLADETPEGRSIVVLAKEKYGI
RGRDMATLNAHFVPFTAQTRMSGVDVDGAVIRKGAVDAILKDLQSSGSAIRPVSPETLRE
VQAIAEEIGKAGGTPLAVARDGKVLGVIYLKDIVKGGIRERFAELRRMGIRTVMITGDNP
LTAAAIAAEAGVDDFLAEATPEAKLKLIRDEQAKGKLVAMCGDGTNDAPALAQADVGVAM
NTGTVAAREAGNMVDLDSDPTKLIEIVEIGKALLMTRGSLTTFSIANDVAKYFAIIPAMF
VAFYPQLGALNVMGLASPQSAILSAIIFNALIIIALIPLALKGVKFRPIGAAALLQRNLL
IYGLGGIVVPFAGIKLIDVAVTALHLV