Protein Info for PS417_15270 in Pseudomonas simiae WCS417

Annotation: membrane protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 286 transmembrane" amino acids 170 to 188 (19 residues), see Phobius details amino acids 238 to 263 (26 residues), see Phobius details TIGR03349: type IV/VI secretion system protein, DotU family" amino acids 60 to 267 (208 residues), 212 bits, see alignment E=4.4e-67 PF09850: DotU" amino acids 62 to 261 (200 residues), 210.8 bits, see alignment E=8.6e-67

Best Hits

KEGG orthology group: K11892, type VI secretion system protein ImpK (inferred from 76% identity to pfs:PFLU3545)

Predicted SEED Role

"Outer membrane protein ImpK/VasF, OmpA/MotB domain"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See U1TP06 at UniProt or InterPro

Protein Sequence (286 amino acids)

>PS417_15270 membrane protein (Pseudomonas simiae WCS417)
MTMDSEYPLDEKTVLLDRDGLGPARGPVTDFPSPPRFEQLEDRMIYAANLPGQRFKMGTN
MLLAAAWELLSQVAQLKASPARESLQALNDQLSSAISAFEAHALHLGAETSQVMSARYVL
CSVIDEAVVTTPWGSCSDWSKRSLLSRFHNETFGGEKFFQLLERLSRDPVKHIAMLELMY
LCLALGFEGKYRVMERGRGQLEAVQDAVYRQIRHVRGDRVPLSISPERGKAPRGRIRIVS
AMWAAVGVLACLLVMYSGFAWVLSQEREAALQPLQFPVSGLSRTPS