Protein Info for GFF2969 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Methylmalonyl-CoA mutase (EC 5.4.99.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01642: MM_CoA_mutase
  • amino acids 23 to 533 (511 residues), 620.1 bits, E=1.5e-190
• TIGR00641: methylmalonyl-CoA mutase N-terminal domain
  • amino acids 36 to 539 (504 residues), 625 bits, E=4.1e-192

Best Hits

KEGG orthology group: K01848, methylmalonyl-CoA mutase, N-terminal domain [EC: 5.4.99.2] (inferred from 87% identity to axy:AXYL_06089)

MetaCyc: 44% identical to 2-hydroxy-isobutanoyl-CoA mutase large subunit (Methylibium petroleiphilum PM1)

Predicted SEED Role

"Methylmalonyl-CoA mutase (EC 5.4.99.2)" in subsystem Propionyl-CoA to Succinyl-CoA Module or Serine-glyoxylate cycle (EC 5.4.99.2)

MetaCyc Pathways

• propanoyl CoA degradation I (3/3 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (13/17 steps found)
• 2-oxobutanoate degradation I (3/4 steps found)
• pyruvate fermentation to propanoate I (4/7 steps found)
• conversion of succinate to propanoate (1/3 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (6/10 steps found)
• 3-hydroxypropanoate cycle (8/13 steps found)
• methylaspartate cycle (12/19 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (11/18 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (7/13 steps found)
• superpathway of L-methionine salvage and degradation (8/16 steps found)
• L-glutamate degradation VIII (to propanoate) (4/11 steps found)
• superpathway of the 3-hydroxypropanoate cycle (9/18 steps found)
• crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered) (3/14 steps found)

KEGG Metabolic Maps

• Propanoate metabolism
• Valine, leucine and isoleucine degradation

Isozymes

Compare fitness of predicted isozymes for: 5.4.99.2

Use Curated BLAST to search for 5.4.99.2

Protein Sequence (541 amino acids)

>GFF2969 Methylmalonyl-CoA mutase (EC 5.4.99.2) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MNRRDPLASTLPAEPELGPISRTGIPVPTMVDASAVNDERIGKPGEYPFTRGIFPDGYQG
RLWTIRQYSGFGTAEESNERYKFLLEKGQTGLSVALDLPTQCGYDPTHPMARPEIGKVGV
SLSNLSEAEILFKDLDLSRISTSFTINGTAAIIYAMYLAVADKQGVPRSKLTGTIQNDIL
KEYVARGTWIFPVRPSMRLIADSILYSNEVTPRFNPISIAGAHVRDAGATAAEEMAYTLA
NGLAYVEELRQRGGDVEKFAKRLSFFFYVHMDFFDEIAKFRAGRRLWARFMKERYGVQDP
KAQHFRFGVVCGGSSLVAPQPYNNAVRVAVETMAAVFGGAQSIFTCAFDEAFQIPTEFSA
ELAVRTQQMIAYETGIARTVDPLGGAYFVEQHTDRMEEMILKVMGEIDAYGGVVPAIEDG
WLQLRLAERGLERKLDTDGGRHVVVGQNYFSKADEKIGVGDVFKLDPTIAQRALEKYQRV
KDTRSQAAVDASLAKLSAAAAQDRENVMPWLVECCHAYATVEEMVDCLKQQWGEFKEPVN
L