Protein Info for PS417_15190 in Pseudomonas simiae WCS417

Annotation: acyl-CoA dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 841 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details transmembrane" amino acids 34 to 61 (28 residues), see Phobius details amino acids 545 to 562 (18 residues), see Phobius details amino acids 591 to 610 (20 residues), see Phobius details PF02771: Acyl-CoA_dh_N" amino acids 128 to 236 (109 residues), 69.9 bits, see alignment E=3.6e-23 PF00441: Acyl-CoA_dh_1" amino acids 363 to 507 (145 residues), 63 bits, see alignment E=5.5e-21 PF09317: ACDH_C" amino acids 517 to 807 (291 residues), 327.2 bits, see alignment E=1.8e-101

Best Hits

Swiss-Prot: 54% identical to FADE_YERPE: Acyl-coenzyme A dehydrogenase (fadE) from Yersinia pestis

KEGG orthology group: K06445, acyl-CoA dehydrogenase [EC: 1.3.99.-] (inferred from 94% identity to pfs:PFLU3529)

Predicted SEED Role

"Butyryl-CoA dehydrogenase (EC 1.3.99.2)" in subsystem Acetyl-CoA fermentation to Butyrate or Anaerobic respiratory reductases or Butanol Biosynthesis or Isobutyryl-CoA to Propionyl-CoA Module or Isoleucine degradation or Valine degradation (EC 1.3.99.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.-, 1.3.99.2

Use Curated BLAST to search for 1.3.99.- or 1.3.99.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7TY31 at UniProt or InterPro

Protein Sequence (841 amino acids)

>PS417_15190 acyl-CoA dehydrogenase (Pseudomonas simiae WCS417)
MVIWLLVGFAAAIALAYRQAVATLWLVTGLIWLAAGYLFNVVAGFGTAVAAILVVAPALL
MTIKPLRRTLLTRRALGLFRTIMPAMSDTERAAIESGTVWWDAELFSGKPNWQRLLQAAP
ASLSAEEQAFLDNEVETLCDIANDWETTQIWQDMSPEGWQYTKDAGFLGMIIPKQYGGKG
FSHYAHSQVVMKLSTRCSAAAISVMVPNSLGPAELLLHYGTDAQRNYYLPRLARGEDIPC
FALTSPYAGSDAGAIPDLGIVCKGTHEGEEVLGFRVTWDKRYITLGPIATVLGLAFRAED
PDGLLGAPGSLGITCALIPTSHPGVNSGRRHWPLNAVFQNGPTTGKDVFIPLEWVIGGRE
QVGNGWRMLMECLAAGRAISLPSANVGLGKVAVRGTTAYAAMRKQFGLPIGKFEGVQAPL
ARMAGHLYACDAVRKVSVASLDAGEKPSVISAIAKYHVTERARIIVNDGMDIVAGKGICM
GPNNFLARAYQQSPIAITVEGANIMTRCLIIFGQGLIRCHPYVFREMEAARNPDRRKALE
AFDSAMFGHVSFVLANTVRAAVHSLTGGRLIAAPAKTEPALASYYRQANRLSVVLALISD
ISMGVLGGALKRKESITGRLGDMLSQLYILSCVLKRFEDDGRPQADLPLVHWSAQDALLR
AHEALAEVFDNYPSKPAAAVVRGLTFPFGIPLRKPSDRLLAQVADVVQTPGETRDRLLAN
SYIPRPEIDKLSYGELGFRLLPQVELIDARLKPAVKQGLIEPMPISATAFTGWRVRARAL
DLISDDEDTLLGRYVEYADHGIQVDDFPQDFGLLEALQQRKQALEPVPKRRTAQGENASV
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