Protein Info for GFF2954 in Variovorax sp. SCN45

Annotation: 6-phosphogluconate dehydrogenase, decarboxylating (EC 1.1.1.44)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00873: 6-phosphogluconate dehydrogenase (decarboxylating)
  • amino acids 6 to 469 (464 residues), 712.6 bits, E=1.1e-218
• PF03446: NAD_binding_2
  • amino acids 7 to 171 (165 residues), 167.7 bits, E=2.2e-53
• PF00393: 6PGD
  • amino acids 179 to 468 (290 residues), 425.6 bits, E=1.1e-131

Best Hits

Swiss-Prot: 58% identical to 6PGD_BACSU: 6-phosphogluconate dehydrogenase, NADP(+)-dependent, decarboxylating (gndA) from Bacillus subtilis (strain 168)

KEGG orthology group: K00033, 6-phosphogluconate dehydrogenase [EC: 1.1.1.44] (inferred from 97% identity to vpe:Varpa_1860)

MetaCyc: 58% identical to 6-phosphogluconate dehydrogenase (decarboxylating) (Bacillus subtilis)
Phosphogluconate dehydrogenase (decarboxylating). [EC: 1.1.1.44]

Predicted SEED Role

"6-phosphogluconate dehydrogenase, decarboxylating (EC 1.1.1.44)" in subsystem Pentose phosphate pathway (EC 1.1.1.44)

MetaCyc Pathways

• superpathway of glucose and xylose degradation (15/17 steps found)
• pentose phosphate pathway (8/8 steps found)
• pentose phosphate pathway (oxidative branch) I (3/3 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of plant hormones
• Glutathione metabolism
• Pentose phosphate pathway

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.44

Use Curated BLAST to search for 1.1.1.44

Protein Sequence (473 amino acids)

>GFF2954 6-phosphogluconate dehydrogenase, decarboxylating (EC 1.1.1.44) (Variovorax sp. SCN45)
MSNPSDFGLIGLAVMGQNLVLNVESRGFQVSVYNRTEATTEAFIAANPGKKLVGAKTLEE
FVQSLAKPRKIQIMVKAGAPVDQVIEQLIPLLDKDDIVIDGGNSLYTDTERRDAYLSSKG
LRFIGAGVSGGEEGARKGPSIMPGGPLSTWEVMKPIFESIAAKVDGDPCVIHIGPGGAGH
YVKMVHNGIEYGDMQLICEAYSLFKAAGFTTDEMAAIFNEWNDGELQSYLIQITAKALEQ
KDPETGKPIVDVILDKAGQKGTGQWTLINAAENAVVISTINAAVEARVLSSQKKARVAAS
KLLQGPKIELSLEKKALVAKVHDALYASKVISYTQGFDLIKTMGDKKDWKLDLGGIASIW
RGGCIIRARFLNRITDAFRTDPALGNLMLDPFFKDLLNRTQQSWREVVALAVSNGIPVPA
FSASLAYYDSYRTERLPANLLQAQRDFFGAHTYERTDKPEGQFFHTDWPEVIG