Protein Info for GFF2950 in Variovorax sp. SCN45

Annotation: Arsenite/antimonite:H+ antiporter ArsB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 430 signal peptide" amino acids 1 to 33 (33 residues), see Phobius details transmembrane" amino acids 48 to 70 (23 residues), see Phobius details amino acids 92 to 110 (19 residues), see Phobius details amino acids 116 to 145 (30 residues), see Phobius details amino acids 152 to 172 (21 residues), see Phobius details amino acids 178 to 198 (21 residues), see Phobius details amino acids 222 to 240 (19 residues), see Phobius details amino acids 246 to 266 (21 residues), see Phobius details amino acids 278 to 296 (19 residues), see Phobius details amino acids 312 to 333 (22 residues), see Phobius details amino acids 375 to 389 (15 residues), see Phobius details amino acids 402 to 425 (24 residues), see Phobius details PF02040: ArsB" amino acids 3 to 422 (420 residues), 505.8 bits, see alignment E=1.3e-155 TIGR00935: arsenite/antimonite efflux pump membrane protein" amino acids 3 to 427 (425 residues), 687.6 bits, see alignment E=4.1e-211 PF03600: CitMHS" amino acids 12 to 368 (357 residues), 206.6 bits, see alignment E=5.6e-65

Best Hits

Swiss-Prot: 76% identical to ARSB_ECOLI: Arsenical pump membrane protein (arsB) from Escherichia coli (strain K12)

KEGG orthology group: K03893, arsenical pump membrane protein (inferred from 87% identity to vpe:Varpa_1865)

MetaCyc: 76% identical to arsenite/antimonite:H+ antiporter (Escherichia coli K-12 substr. MG1655)
RXN-22385; RXN3O-9786

Predicted SEED Role

"Arsenic efflux pump protein" in subsystem Arsenic resistance

MetaCyc Pathways

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (430 amino acids)

>GFF2950 Arsenite/antimonite:H+ antiporter ArsB (Variovorax sp. SCN45)
MPIAVLIFVVTLVLVIWQPRGLGIGWSASLGAAVALLLGVVQPADIPVVWGIVWNATATF
VAVIVISLLLDEAGFFEWAALHVARWGGGRGRRLFALVVLLGAAVSALFANDGAALILTP
IVMAMLAALGFTPAATLAFVMAAGFIADTASLPLIVSNLVNIVSADFFRIGFGAYASVMV
PVNIAAVLASLAVLMLYFRRGIPARYDAARLKAPADAIRDPATFRAGWVVLALLLLGFFG
LEPLGVPVSAVAAFGAVVLLAVAGRGPVIGIRGVLRGAPWQIVVFSLGMYLVVYGLRNAG
LTGQLAALLDVFAQGGIWGAAMGTGFLAAVLSSVMNNMPTVLVGALSIDASAASGAVKEA
MVYANVIGCDLGPKITPIGSLATLLWLHVLAKKGTVIGWGGYFRVGIVLTLPVLFVTLAA
LAFRLSIPLP