Protein Info for GFF2948 in Xanthobacter sp. DMC5

Annotation: Sensor histidine kinase RcsC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 770 transmembrane" amino acids 41 to 62 (22 residues), see Phobius details amino acids 200 to 222 (23 residues), see Phobius details TIGR00229: PAS domain S-box protein" amino acids 262 to 361 (100 residues), 25.7 bits, see alignment E=5.1e-10 PF08447: PAS_3" amino acids 262 to 340 (79 residues), 43 bits, see alignment E=1.4e-14 PF13188: PAS_8" amino acids 368 to 407 (40 residues), 19.2 bits, see alignment 2.8e-07 PF12860: PAS_7" amino acids 373 to 479 (107 residues), 56.9 bits, see alignment E=7e-19 PF00512: HisKA" amino acids 529 to 598 (70 residues), 66.8 bits, see alignment E=4.4e-22 PF02518: HATPase_c" amino acids 643 to 754 (112 residues), 96.4 bits, see alignment E=4.3e-31

Best Hits

KEGG orthology group: K07716, two-component system, cell cycle sensor histidine kinase PleC [EC: 2.7.13.3] (inferred from 92% identity to xau:Xaut_1626)

Predicted SEED Role

"Two-component sensor histidine kinase PleC" in subsystem Orphan regulatory proteins

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (770 amino acids)

>GFF2948 Sensor histidine kinase RcsC (Xanthobacter sp. DMC5)
MARANAASVGARDQAMRGLARSIARPAYQRLLDAEPVLRRIVPALIVTFLGVIGVGAVVQ
VHAHRVAELNNAKDDIALLALATADALTLRAGNSLAKVPGALAESLPPRATEAGRRVFVT
DGQGHVVAAAPPGEAPANLSDILDPSQPLVIFAERAGVLEVPYGTGTALATVRNLQAPLG
QIVYLQPTEKALAAWSDTTTLTVTLFTTTGAVLLILGFSFHWQASRAREADVIYETVRRR
IDTALNRGRCGMWDWDMARGRMYWSDTMFEILGLEHRDELVSFGEISRLVHPDDGDLYEL
AQELAERPGTPVDRVFRMRTAKGDFVWLRMRAEVVQQAGEDGPHLIGIAMDVTEAQRMAE
RTVTADMRLRDAIESISEAFVLWDSQNRLVLCNSKFQSLHELPDETIVAGTPFDTIASVG
RHPVTRTPLKPEDKPEEGSRAFEAQLSNGRWLKIAERRTKDGGYVSVGTDITTMKRHEER
LMDNEKRLMALVADLRKSQQTLEHQAQQLAELAEKYSEERNKAEDANRAKSEFLANMSHE
LRTPLNAIIGFSEIMESGMFGPLGSAKYAEYCSDIKGSGTYLLDVINDILDMSKIEAGRM
QLEVEDVALGEIIADAMRVTAVNGDEKGVAMTMEAADNLVIKGDRRALKQILLNLLSNAV
KFTPEGGRIAVKARVNGAAAVIAIEDTGIGIAKGALARIGRPFEQVESQFTKTHKGSGLG
LAIAKSLVELHGGAMRIRSIEGSGTTVFVRLPVDGRAANSNETPRLAAVM