Protein Info for GFF2945 in Variovorax sp. SCN45

Annotation: Possible glyoxylase family protein (Lactoylglutathione lyase) (EC 4.4.1.5)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00903: Glyoxalase
  • amino acids 2 to 127 (126 residues), 76 bits, E=3.4e-25
• PF13669: Glyoxalase_4
  • amino acids 4 to 111 (108 residues), 44.9 bits, E=1.3e-15

Best Hits

Swiss-Prot: 51% identical to YYAH_BACSU: Uncharacterized protein YyaH (yyaH) from Bacillus subtilis (strain 168)

KEGG orthology group: K01759, lactoylglutathione lyase [EC: 4.4.1.5] (inferred from 97% identity to vpe:Varpa_1870)

Predicted SEED Role

"Possible glyoxylase family protein (Lactoylglutathione lyase) (EC 4.4.1.5)" (EC 4.4.1.5)

MetaCyc Pathways

• methylglyoxal degradation VIII (3/3 steps found)
• superpathway of methylglyoxal degradation (6/8 steps found)
• methylglyoxal degradation I (2/3 steps found)

KEGG Metabolic Maps

• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.4.1.5

Use Curated BLAST to search for 4.4.1.5

Protein Sequence (129 amino acids)

>GFF2945 Possible glyoxylase family protein (Lactoylglutathione lyase) (EC 4.4.1.5) (Variovorax sp. SCN45)
MRIDHIALWTTDLERCKRFYVDYFGATAGAGYVNPAKGFASCFLSLGHGARIEAMTTSTL
SPVTAEAGAQRMGWTHLAISVGSDAAVDALTQRLKADGYPLLDGPRRTGDGYYESVVLDP
DGNRIEITA