Protein Info for GFF2931 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 584 TIGR01417: phosphoenolpyruvate-protein phosphotransferase" amino acids 5 to 577 (573 residues), 505 bits, see alignment E=1.3e-155 PF05524: PEP-utilisers_N" amino acids 6 to 133 (128 residues), 113 bits, see alignment E=1.5e-36 PF00391: PEP-utilizers" amino acids 169 to 242 (74 residues), 84.6 bits, see alignment E=4.5e-28 PF02896: PEP-utilizers_C" amino acids 268 to 560 (293 residues), 356.6 bits, see alignment E=1.5e-110

Best Hits

KEGG orthology group: K08483, phosphotransferase system, enzyme I, PtsI [EC: 2.7.3.9] (inferred from 77% identity to pol:Bpro_0299)

Predicted SEED Role

"Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9)" in subsystem Fructose and Mannose Inducible PTS or Fructose utilization or Mannitol Utilization (EC 2.7.3.9)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.3.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (584 amino acids)

>GFF2931 Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MTISIHGLAVSRGIAIGRAVIVASSRVDVAHYFVQADQVEREIERLRQARNAVAEEIVKV
QQSLHELGPNDAHPELTALLDVHLMLLQDEQLTSGVKHWIVDRHYNAEWALTTQLEVIAR
QFDEMEDPYLRERKADLEQVVERMLRFMRGVASPVAPGGETRTPQDSAIEAPLVLIAHDL
SPADMLQFKQSVFAGFVTDVGGKTSHTAIVARSMDIPAVVGARSSSQLIQQDDWVIIDGD
AGVVLVDPSPILLAEYGFKQRQGEVERERLSRLKNTPAVTLDGQKIELLANIEQPEDAVA
ALKAGAVGVGLFRSEFLFMGRAGKLPDEEEQYQAYRRAVEGMQGLPVTIRTVDVGADKPL
DRGASRAADDHLNPALGLRAIRWSLAEPAMFLVQLRAILRAAAHGPVHLLIPMLAHASEI
RQTLALVNRAREQLDNAGVAYGHVKLGAMIEVPAAALTIPLFLRYFDFLSIGTNDLIQYT
LAIDRADEAVSHLYDPVHPAVLQLLASTIEQCRAKGKGVSVCGEMAGDVSLTRLLLGLGL
RTFSMHPSQILAVKQQILRCDTTRLQTWAQSVLAAEDPAALMTD