Protein Info for GFF2928 in Pseudomonas sp. DMC3

Annotation: Glyoxylate/hydroxypyruvate reductase B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 322 PF00389: 2-Hacid_dh" amino acids 5 to 319 (315 residues), 103.3 bits, see alignment E=1.2e-33 PF02826: 2-Hacid_dh_C" amino acids 108 to 287 (180 residues), 214.8 bits, see alignment E=9.1e-68 PF03446: NAD_binding_2" amino acids 148 to 258 (111 residues), 25.7 bits, see alignment E=1.6e-09

Best Hits

Swiss-Prot: 59% identical to GHRB_KLEP7: Glyoxylate/hydroxypyruvate reductase B (ghrB) from Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)

KEGG orthology group: K00090, gluconate 2-dehydrogenase [EC: 1.1.1.215] (inferred from 91% identity to pfo:Pfl01_2904)

MetaCyc: 83% identical to 2-dehydro-6-phosphogluconate reductase (Pseudomonas fluorescens)
Phosphogluconate 2-dehydrogenase. [EC: 1.1.1.43]

Predicted SEED Role

"2-ketogluconate 6-phosphate reductase (EC 1.1.1.43)" in subsystem 2-Ketogluconate Utilization (EC 1.1.1.43)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.215

Use Curated BLAST to search for 1.1.1.215 or 1.1.1.43

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (322 amino acids)

>GFF2928 Glyoxylate/hydroxypyruvate reductase B (Pseudomonas sp. DMC3)
MKKKVVLYKKLSPALMARLQEQFDVTLIESLDADGVMQLRDALPRAHGLLGASLKLDAAL
LDLAPQLEAIASVSVGVDNYDIEYLNRRKILLTNTPDVLTETTADTGFALILAAARRVVE
LANMVRGGQWSRNIGPAHFGTDVHGKTLGIIGMGRIGEALAQRGHFGFGMPVIYHSQSRK
PAVEERFEAQYRSLDELLQQADFICLTLPLTAQTERLIGAEQFALMRPESIFINISRGKV
VDEAAMIEALRSKRIRAAGLDVFEREPLNHDSPLLQLSNVVATPHMGSATHETREAMARC
AVENLLAALNGQRPANLVNPLN