Protein Info for GFF2926 in Pseudomonas sp. DMC3

Annotation: 2-dehydro-3-deoxygluconokinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00294: PfkB
  • amino acids 5 to 303 (299 residues), 209 bits, E=5.2e-66

Best Hits

Swiss-Prot: 52% identical to KDGK_BACSU: 2-dehydro-3-deoxygluconokinase (kdgK) from Bacillus subtilis (strain 168)

KEGG orthology group: K00874, 2-dehydro-3-deoxygluconokinase [EC: 2.7.1.45] (inferred from 85% identity to pfo:Pfl01_2902)

MetaCyc: 79% identical to 2-ketogluconokinase (Pseudomonas fluorescens)
Dehydrogluconokinase. [EC: 2.7.1.13]

Predicted SEED Role

"2-ketogluconate kinase (EC 2.7.1.13)" in subsystem 2-Ketogluconate Utilization (EC 2.7.1.13)

MetaCyc Pathways

• glucose degradation (oxidative) (5/5 steps found)
• Entner-Doudoroff pathway III (semi-phosphorylative) (7/9 steps found)
• D-fructuronate degradation (3/4 steps found)
• superpathway of β-D-glucuronosides degradation (4/7 steps found)
• 4-deoxy-L-threo-hex-4-enopyranuronate degradation (2/5 steps found)
• D-galacturonate degradation I (2/5 steps found)
• 3,6-anhydro-α-L-galactopyranose degradation (3/7 steps found)
• alginate degradation (2/7 steps found)
• superpathway of hexuronide and hexuronate degradation (4/10 steps found)
• superpathway of microbial D-galacturonate and D-glucuronate degradation (14/31 steps found)

KEGG Metabolic Maps

• Pentose and glucuronate interconversions
• Pentose phosphate pathway

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.45

Use Curated BLAST to search for 2.7.1.13 or 2.7.1.45

Protein Sequence (335 amino acids)

>GFF2926 2-dehydro-3-deoxygluconokinase (Pseudomonas sp. DMC3)
MSDIDILSFGETMAMLVAEQTGDLAKVEQFHKRIAGADSNVAIGLSRLGFNVAWLSRVGN
DSLGRFVVETLIKEGLDCSHVEVDKNHPTGFQFKSRNDDGSDPQVEYFRRGSAASHLSPH
AINDNLLSARHLHATGIPPALSASAREMAVELMTRMRAAGRSVSFDPNLRPSLWASEAEM
IREINRLAALAHWVLPGLSEGRLLSGFDDPADIAAFYLDQGAEAVAIKLGPEGAYYRTQL
DQGFVAGVPVSNVVDTVGAGDGFAVGMISALLEHQSFAEAVQRANWIGSRAVQSRGDMEG
LPSRLELTAEFASRLAPTGERISHAGASLLAKRPA