Protein Info for PS417_14925 in Pseudomonas simiae WCS417

Annotation: 2-methylcitrate dehydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 449 PF03972: MmgE_PrpD_N" amino acids 5 to 246 (242 residues), 233.7 bits, see alignment E=2e-73 PF19305: MmgE_PrpD_C" amino acids 267 to 439 (173 residues), 159.4 bits, see alignment E=8.1e-51

Best Hits

KEGG orthology group: None (inferred from 92% identity to pfs:PFLU3474)

Predicted SEED Role

"MmgE/PrpD family protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7ULQ8 at UniProt or InterPro

Protein Sequence (449 amino acids)

>PS417_14925 2-methylcitrate dehydratase (Pseudomonas simiae WCS417)
MSHTQALCRFLAELNVAQLPEEVLARTEDLYLDWLASALASKGAHPIPLFERYAQKMGPG
SGPAQVIVNGGSSSAYFAALVNAASSHLVEQDDLHNSSVLHPATVVFPAALAAAQDLGKS
GSDLLLASVAGYEAGIRIGEFMGRSHYRIFHTTATVGTLAAAVAVGKLLDFNEEQFINLL
GSAGTQAAGLWEFLRDAADSKQLHTAKAAADGLLAAYLTADGLTGARNILEGDQGLAAGM
STDAEPARLSADLGSRWALLETSFKFHASCRHTHPAADALLDLMQREGLHAEQIAHVETR
VHQGAIDVLGRVTVPASVHQAKFSMGTVLGLIAVHGKAGLTEFHELALTDPAVAALRDKV
SMTLDPEVDGAYPQRWLGRVTVTTLEGRTLHGAIDEPKGDPGNTLSRAELADKFQRLTQF
SNARTPAQANALIDKVWDLRNAVSMADWL