Protein Info for GFF2899 in Pseudomonas sp. DMC3
Annotation: Polyol:NADP oxidoreductase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 48% identical to M2DH_ASPFC: Mannitol 2-dehydrogenase (AFUB_071700) from Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163)
KEGG orthology group: None (inferred from 92% identity to pfo:Pfl01_2636)MetaCyc: 42% identical to D-mannonate oxidoreductase (Escherichia coli K-12 substr. MG1655)
Fructuronate reductase. [EC: 1.1.1.57]
Predicted SEED Role
"Multiple polyol-specific dehydrogenase (EC 1.1.1.-)" in subsystem Mannitol Utilization or Ribitol, Xylitol, Arabitol, Mannitol and Sorbitol utilization (EC 1.1.1.-)
MetaCyc Pathways
- D-fructuronate degradation (3/4 steps found)
- mannitol cycle (3/5 steps found)
- superpathway of β-D-glucuronosides degradation (4/7 steps found)
- superpathway of hexuronide and hexuronate degradation (4/10 steps found)
- superpathway of microbial D-galacturonate and D-glucuronate degradation (14/31 steps found)
KEGG Metabolic Maps
- Alkaloid biosynthesis I
- Aminosugars metabolism
- Ascorbate and aldarate metabolism
- Benzoate degradation via CoA ligation
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of type II polyketide products
- Biosynthesis of unsaturated fatty acids
- Bisphenol A degradation
- Butanoate metabolism
- C21-Steroid hormone metabolism
- Fructose and mannose metabolism
- Glycine, serine and threonine metabolism
- Insect hormone biosynthesis
- Linoleic acid metabolism
- Nucleotide sugars metabolism
- Pentose and glucuronate interconversions
- Polyketide sugar unit biosynthesis
- Retinol metabolism
- Tetrachloroethene degradation
Isozymes
Compare fitness of predicted isozymes for: 1.1.1.-
Use Curated BLAST to search for 1.1.1.- or 1.1.1.57
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (490 amino acids)
>GFF2899 Polyol:NADP oxidoreductase (Pseudomonas sp. DMC3) MKLNQQNLNRLAAQVQLPGYNLSDTCQGIAHIGVGGFHRAHQAYYTDALMNTGEALDWAI CGVGLRPEDRRARDDLREQDYLFTLFELGDSDDTEVRVIGAIRDMLLAEDGAQTLIDKLA EPQIRIVSLTITEGGYCIDDSSGEFMAHLPQIQHDLAHPEAPGTVFGFLCAALAKRRAAG VPAFTVMSCDNLPHNGAVARKALLAFAALRDPDLRDWIDQNVSFPNAMVDRITPMTSTAH RLQLADKHGVDDAWPVVCEPFVQWVLEDKFVNGRPAWEKVGVQFTDDVSPYEEMKIKLLN GSHLALTYLGFLKGYRFVHETMNDPLFVRYMRAYMDLDVTPQLAPVPGIDLSEYKDTLVA RFSNQAIADQLERVCSDGSSKFPKFTVPTINRLIADGRETKRAALVVAAWALYLKGVDEN GDTYSIPDPRAAFCQGLVADEGLTKERLLGVEEIFGTAIPKSAEFVAAFEWCCQSLREVG VSRTLERVLG