Protein Info for GFF2878 in Variovorax sp. SCN45

Annotation: DNA primase DnaG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 681 TIGR01391: DNA primase" amino acids 9 to 430 (422 residues), 450.5 bits, see alignment E=2.7e-139 PF01807: zf-CHC2" amino acids 10 to 104 (95 residues), 128.7 bits, see alignment E=1.9e-41 PF08275: DNAG_N" amino acids 135 to 264 (130 residues), 124.4 bits, see alignment E=9.6e-40 PF13662: Toprim_4" amino acids 272 to 345 (74 residues), 58.6 bits, see alignment E=1.8e-19 PF01751: Toprim" amino acids 274 to 345 (72 residues), 38.3 bits, see alignment E=3.8e-13 PF13155: Toprim_2" amino acids 275 to 362 (88 residues), 61.3 bits, see alignment E=2.8e-20 PF10410: DnaB_bind" amino acids 381 to 434 (54 residues), 43.1 bits, see alignment 1.2e-14

Best Hits

KEGG orthology group: K02316, DNA primase [EC: 2.7.7.-] (inferred from 90% identity to vap:Vapar_1763)

Predicted SEED Role

"DNA primase (EC 2.7.7.-)" in subsystem DNA-replication or Macromolecular synthesis operon (EC 2.7.7.-)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (681 amino acids)

>GFF2878 DNA primase DnaG (Variovorax sp. SCN45)
LPLFAYMTIPASFIQELIARADVVEIVGRYVPLKKAGANFMGLCPFHGEKSPSFSVSPTK
QFYHCFGCGVHGNAIGFLMEHAGMGFVEAVHDLADQYGLQVPEDDASPAERARAASQRQK
QATLTDVLEKAGDAYRKHLRQAPGAIEYLKGRGVSGEVAKQFGIGYAPAGWRALASVFPD
YDDPLLTESGLVIVNNEEGKLSDEEAKRYDRFRDRVMFPIRNVKGECIGFGGRVLGDEKP
KYLNSPETPVFSKGRELYGLYEARAAFRDRGYALVTEGYMDVVALAQLGFPNAVATLGTA
CTSEHVQKLFRFTESVVFSFDGDAAGRRAARKALDGALPYATDVRSIKFLFLPAEHDPDS
FIREHGAEAFARFVTEATPLSRFMLEAAREGCDLTTAEGRAHMTSNVRPLWSAMPDGALK
RQLLSEIATLVQLDAPALSELWASTPGPRKAGGGALAAQRHNEPPMYTGGDGDDYYPDMP
PPQDYEPPRYESDSKPKFQPQRKFTKGKRWGGKFEEPIEPLRGRGAPPSRPDVAVRLLLS
NMAQWEALSHELHLMLCELPGSHGALFTWLDSQLHEHGVQPWAALREGLRGLDFEPLAER
LMAVSEGGPVPEGEEEQHLADAAKELTSVLDFMLDDRLKAQQSEAIAAVGKDPKALERYK
ALEARRLELRNRLRPADAEGA