Protein Info for GFF2877 in Hydrogenophaga sp. GW460-11-11-14-LB1
Annotation: Methylglyoxal reductase, acetol producing (EC 1.1.1.-) / 2,5-diketo-D-gluconic acid reductase B (EC 1.1.1.274)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 63% identical to DKGB_SALTY: 2,5-diketo-D-gluconic acid reductase B (dkgB) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
KEGG orthology group: K06222, 2,5-diketo-D-gluconate reductase B [EC: 1.1.1.274] (inferred from 71% identity to aaa:Acav_0345)MetaCyc: 63% identical to methylglyoxal reductase DkgB (Escherichia coli K-12 substr. MG1655)
Aryl-alcohol dehydrogenase (NADP(+)). [EC: 1.1.1.91]; RXN0-7020 [EC: 1.1.1.91, 1.1.1.346]; Aldehyde reductase. [EC: 1.1.1.91, 1.1.1.346, 1.1.1.21]
Predicted SEED Role
"Methylglyoxal reductase, acetol producing (EC 1.1.1.-) / 2,5-diketo-D-gluconic acid reductase B (EC 1.1.1.274)" in subsystem D-gluconate and ketogluconates metabolism (EC 1.1.1.-, EC 1.1.1.274)
MetaCyc Pathways
- methylglyoxal degradation III (1/2 steps found)
- ketogluconate metabolism (5/8 steps found)
- superpathway of methylglyoxal degradation (4/8 steps found)
- L-ascorbate biosynthesis VIII (engineered pathway) (3/7 steps found)
- detoxification of reactive carbonyls in chloroplasts (1/10 steps found)
- superpathway of pentose and pentitol degradation (8/42 steps found)
KEGG Metabolic Maps
- Alkaloid biosynthesis I
- Aminosugars metabolism
- Ascorbate and aldarate metabolism
- Benzoate degradation via CoA ligation
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of type II polyketide products
- Biosynthesis of unsaturated fatty acids
- Bisphenol A degradation
- Butanoate metabolism
- C21-Steroid hormone metabolism
- Fructose and mannose metabolism
- Galactose metabolism
- Glycerolipid metabolism
- Glycine, serine and threonine metabolism
- Insect hormone biosynthesis
- Linoleic acid metabolism
- Nucleotide sugars metabolism
- Pentose and glucuronate interconversions
- Polyketide sugar unit biosynthesis
- Pyruvate metabolism
- Retinol metabolism
- Tetrachloroethene degradation
Isozymes
Compare fitness of predicted isozymes for: 1.1.1.-
Use Curated BLAST to search for 1.1.1.- or 1.1.1.21 or 1.1.1.274 or 1.1.1.346 or 1.1.1.91
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (270 amino acids)
>GFF2877 Methylglyoxal reductase, acetol producing (EC 1.1.1.-) / 2,5-diketo-D-gluconic acid reductase B (EC 1.1.1.274) (Hydrogenophaga sp. GW460-11-11-14-LB1) MLNHPIPAFGLGTFRLQGQVVIDAVRQALELGYRHIDTAQIYGNEAEVGQAIAESGVPRG ELFVTTKVWTEHLAADRLVPSLQESLRKLRLEQVDLALIHWPSPGGAVPLADSLHALQAA QAQGLTAHIGVSNFTVPLLRQAIDTVGAKAIATNQIELHPWLQSPKLVAFAREQGIHLTS YMTLAYGQVLGDPVITEIARERGWTPAQVVLAWALQRGFSVIPSSTQRENLQGNLLAPTL RLNDTEMARIDALDRGQRLVDPKGLAPAWD