Protein Info for GFF2848 in Pseudomonas sp. DMC3

Annotation: Extracellular serine protease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1025 signal peptide" amino acids 1 to 33 (33 residues), see Phobius details PF00082: Peptidase_S8" amino acids 73 to 408 (336 residues), 151.6 bits, see alignment E=4.6e-48 TIGR02601: autotransporter-associated beta strand repeat" amino acids 483 to 514 (32 residues), 42.2 bits, see alignment (E = 5e-15) PF12951: PATR" amino acids 484 to 513 (30 residues), 41.8 bits, see alignment (E = 1.3e-14) TIGR01414: outer membrane autotransporter barrel domain" amino acids 548 to 1025 (478 residues), 183 bits, see alignment E=1e-57 PF03797: Autotransporter" amino acids 753 to 1005 (253 residues), 208.3 bits, see alignment E=2.7e-65

Best Hits

KEGG orthology group: K12685, subtilase-type serine protease [EC: 3.4.21.-] (inferred from 92% identity to pfo:Pfl01_2684)

Predicted SEED Role

"Serine protease homologue"

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.-

Use Curated BLAST to search for 3.4.21.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1025 amino acids)

>GFF2848 Extracellular serine protease (Pseudomonas sp. DMC3)
MDNNNTPAQSGGRFALKTLTVAMLCASAMAQAAPYVENGHNGDPSSWRSNEFKADWGLGA
IGADYAYAAGYTGKGVKLGIFDQPVYAAHPEFSGSNKVVTLVTSGIREYTDPYIPVKAGD
AFRYDGSPSVGSDGKLGAHGTHVGGIAAGSRDGGVMHGVAFGAQIISADNGDPGPEDGIV
RGNDGAVYKAGWDALIASGARIINNSWGIGITDRFDLGGRDPAYPHFTVNDAQLQFNEIR
TLLGTKPGGAYDGAIAAARSGIVTIFAAGNDYNLNNPDAIAGLGYFVPDIAPNWITVAAL
QQNPDTASANPYVISTFSSRCGYTASYCVSAPGTKIYSSIIEGTNVDNLTTGYKNYNGTS
MAAPHVAGSIAVLMERFPYMSGDQVASVLRTTATDLGAPGIDALYGWGMINLRKAIDGPA
MFVTEQDIPEEFRVQGAYGSGQFVADLPGIGAIIDQGKPTERVCSDITCGLDTWRNDIGG
HGGLTKQGIGTLVLTGNSTYSGPTLVNQGRLAINGSLQSAVTVNNGGILGGNGRIGALAV
NSGGTVAPGNSIGTLNVAGDVTFAPGSTYAVELSPTSSDQIVAGGKAVIDGATVSLSLEN
SPTLLSTGEVQSLLGTRYNILQAAGGIEGRFGQVLPNYAFLGGTLDYSAGGIQLAVGRNE
ASFASVGLTPNQRAVGAAAERLGAGNALFETLLLSPNAASAQQAFQQLSGEIHPAIGTLL
INDSRYLREAVGERLRERDLFNADAPTDERSNVWVKVLGSWGESDGGHDNADSNSSIGGL
LAGVDGLIAEDTRLGFVTGYSDSSLSMGSGTHSSASVDSYHLGAYLGHEIDALRLTVGGA
YSWHRIDVKRDLQFGDVSGKQKTKRDATTAQLFTEAAYDLGLQPMNLEPFANLSYVHLNT
DSFTEKGDAAALKGGEDNRDVVLSTLGVRAKRSFAVSDKHQLELGASLGWQHNLSSVDAD
SHLAFANGNSAFTVQSVSMDRDAAVVGVRAGLALNRDVRVNLDYNGLIGSNEKDHGVGLT
LDWQF