Protein Info for GFF2832 in Sphingobium sp. HT1-2

Annotation: Maltodextrin glucosidase (EC 3.2.1.20)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 532 PF00128: Alpha-amylase" amino acids 15 to 462 (448 residues), 405.2 bits, see alignment E=5.1e-125 PF11941: DUF3459" amino acids 445 to 514 (70 residues), 30.7 bits, see alignment E=3.4e-11

Best Hits

Swiss-Prot: 53% identical to AGLA_RHIME: Probable alpha-glucosidase (aglA) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K01187, alpha-glucosidase [EC: 3.2.1.20] (inferred from 80% identity to sjp:SJA_C1-11350)

Predicted SEED Role

"Maltodextrin glucosidase (EC 3.2.1.20)" in subsystem Maltose and Maltodextrin Utilization (EC 3.2.1.20)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.20

Use Curated BLAST to search for 3.2.1.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (532 amino acids)

>GFF2832 Maltodextrin glucosidase (EC 3.2.1.20) (Sphingobium sp. HT1-2)
MTDTALWWRGATIYQIYPRSFADSNGDGIGDLAGITAHLPYVASLGVDAIWLSPFFKSPM
RDFGYDVSDYCDVDPIFGTLADFDALIARAHDLGLRIIVDQVWAHTSDEHPWFIESRSSR
QNDHADWYVWADAKPDGTPPNNWQSVFGGPAWTWDARRGQYYMHNFLKEQPQINLHKPQV
QAAVLDIVRFWLDRGVDGFRIDAINHSMPDPQLRDNPPAPEDGRIRTRPYDFQIKRYSQS
HPDIPLFLEKVRSVFDEYPDRFTVAEVGGDDSDAEMKAFTQGDHRLNTAYGFDFLYAPKL
TAPFLKDALSRWPAEQGIGWPSWAFENHDAPRAVSRWAGDIDAHAYCRMKMLLLACLRGN
IFLYYGEELGLPQVDIAFEDLQDPEAIANWPLTLSRDGARTPMPWTGAAPWLGFSDAKPW
LPVGEAHRPLAVDAQEADPASLLHWTREVLALRNATPALRSGTITFLDTPDDLLAFERTQ
DGQQRLCVFNLGTGPVAWRPADADRWQVELSTGTIADWNFAPASGLVAAFQV