Protein Info for GFF2830 in Pseudomonas sp. DMC3

Annotation: Selenocysteine-specific elongation factor

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 638 TIGR00475: selenocysteine-specific translation elongation factor" amino acids 1 to 627 (627 residues), 483.7 bits, see alignment E=8.7e-149 PF00009: GTP_EFTU" amino acids 2 to 164 (163 residues), 118 bits, see alignment E=1.2e-37 TIGR00231: small GTP-binding protein domain" amino acids 3 to 144 (142 residues), 43.9 bits, see alignment E=2.2e-15 PF01926: MMR_HSR1" amino acids 3 to 107 (105 residues), 23.4 bits, see alignment E=1.6e-08 PF03144: GTP_EFTU_D2" amino acids 192 to 260 (69 residues), 33.5 bits, see alignment E=1.4e-11 PF09106: SelB-wing_2" amino acids 442 to 498 (57 residues), 52.9 bits, see alignment 9.5e-18 PF21214: SelB_WH_2nd_bact" amino acids 507 to 563 (57 residues), 47.7 bits, see alignment 3.5e-16 PF09107: SelB-wing_3" amino acids 582 to 625 (44 residues), 59.8 bits, see alignment 4.4e-20

Best Hits

KEGG orthology group: K03833, selenocysteine-specific elongation factor (inferred from 83% identity to pfo:Pfl01_2707)

Predicted SEED Role

"Selenocysteine-specific translation elongation factor" in subsystem Selenocysteine metabolism

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (638 amino acids)

>GFF2830 Selenocysteine-specific elongation factor (Pseudomonas sp. DMC3)
VIVGTAGHIDHGKTSLLQALTGQTGDRRPQERERGMTIDLGYLYAELAAGAGLTGFIDVP
GHEKFTHNMLAGAQGIDLVLLVVAADDGVMPQTREHLAIVELLGIPRALVAITKCDRAGA
GRVEEVQRQVLDLLAPGPFADAPIFTVSSVNGQGVGELRAALLQAQNEVQARGCEGGFRL
AIDRAFSVAGAGIVVTGTALSGTVAVGDKLVLGPAGKSVRIRGLHAQNQAAEQAFAGQRV
ALNLSSDRLELAQIHRGQWLLADWLHAPTQRIDIDFQLLPGERTFEHFHPVHLHLGTQDV
IARVALLQGPRLPPGERMFAQLLINAPVQAVKGDRLILRDSSAQRTLGGGVVLDPFAPAR
QRRSPERLAQLQTLALSVSLEQALPALLNQSPGGLDPQRLERQFNRRRAQWSLAENIRLI
DTRQGPLLFNVQRWGRLKFTLLEQLARFHEQEPDQMGPDRDRLRRFSALALERSTFISLL
DELLSAGSILASGPWLHLPDHQVRLNADDEILWQQLQPLFEQAGFDPPWVRDAAKMLGED
DASVRLLMRKLARLGLMHQVVRDLFISDIVLRQMAALLLKLAEENPAIEVTTFRDALGLG
RKRCIQYLEYFDRLGLTRRLGESRQIRPDNALADASAP